不同种属动物消化道内大豆凝集素免疫活性残留的比较研究  

Comparative Study on Immunological Activity Survival of Soybean Agglutinin in Digestive Tracts of Different Species of Animals

作　　者：王利民[1] 胡海霞[1] 王涛[1] 秦贵信[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《畜牧兽医学报》2008年第5期682-686,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目资助(C020302)

摘　　要：比较研究大豆凝集素(SBA)在反刍动物(羊)、肉食动物(狗)、啮齿动物(兔)、杂食动物(猪)和禽类(鸡)等不同种属动物消化道不同区段食糜中的免疫活性的残留规律,并探讨SBA对不同种属动物抗营养机制的差异。各种属动物均选取半性成熟雄性8只(头)。日粮中含20%的生大豆和质量分数1%的Cr2O3(外源指示剂),试验期为2周,最后一次进食2 h后,麻醉状态下颈动脉放血,立即取十二指肠、空肠前、空肠中、空肠后、回肠、盲肠、结肠(鸡为结直肠)各肠段内的食糜。食糜和饲料中具有免疫活性的SBA采用竞争性间接抑制ELISA方法检测,食糜和饲料中的Cr采用原子吸收光谱法检测。结果表明,同一种动物不同肠段内的SBA残留率不同,总的趋势是由十二指肠到结肠呈下降趋势,其中由十二指肠到空肠前段的残留率变化均达到显著水平(P<0.05),各肠段内均能检测到SBA。在不同动物的同一区段肠道内,SBA的残留率随动物的不同而不同,各肠段中SBA的残留率在兔均最低;十二指肠到空肠SBA的残留率在羊和鸡较高、在猪和狗居中;空肠之后在猪、羊和鸡较高。SBA在不同种属动物及其消化道不同部位内的免疫活性残留规律不同,SBA对不同种属动物的抗营养机制和抗营养作用可能不尽相同。In this experiment, different animals （sheep as ruminants, dog as carnivores, rabbit as rodents, pig as omnivores and chicken as fowls） were used to compare the immunological activity survival disciplinarian of SBA in the chyme within different parts of digestive tracts. Each animal aged the half of their sexual maturity, 8 each species with similar conditions （sex） were looked as a repeat and fed the diet containing 20% raw soybean and 1%Cr203（ as exogenous indicator）. Two hours after the last meal of two weeks＇ feeding, the chymes in duodenum, upper-jejunum, middle-jejunum, lower-jejunum, ileum, appendix and colon were taken. The SBA having immunological activity in the chymes and feeds were detected with ELISA and the Cr in the chymes and feeds were detected with the absorption spectroscopy. The survival rates of SBA in different parts of digestive tracts in the same animal were different and showed the downtrend from duodenum to colon, however, the SBA were existent in all of the parts of digestive tracts. The changes of the survival rate from duodenum to front-jejunum were significant（P〈0.05）in all of the animals.There were different survival rates in the same parts of digestive tracts in the different animals. Rabbits possesed the lowest survival rates in all of the digestive parts. Sheep and chickens were higher than pigs and dogs from duodenum to front-jejunum. Behind jejunum, pigs, sheep and chicken had more survival rates than that of others. There are different immunological activity survival disciplinarian of SBA in different animals and their different parts of intestinal tracts. The antinutritional mechanism and effects of SBA on different species of animals maybe different.

关 键 词：消化道 食糜 大豆凝集素(SBA) 免疫活性 残留 

分 类 号：S816.42[农业科学—饲料科学]