硅橡胶膜生物反应器生物转化制备香草醛  

Preparation of Vanillin by Bioconversion in a Silicon Rubber Membrane Bioreactor

作　　者：伍云涛[1] 冯明[1] 丁文武[1] 汤晓玉[1] 钟月华[1] 肖泽仪[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《四川大学学报（工程科学版）》2008年第3期91-95,共5页Journal of Sichuan University (Engineering Science Edition)

基　　金：国家自然科学基金资助项目(2027604120776088)

摘　　要：在硅橡胶膜生物反应器(SRMBR)系统中用丁香油为原料和大豆酯氧化酶(SBLOX)为催化剂,进行了生物转化制备香草醛的实验研究。与摇瓶发酵实验相比,在SRMBR中的发酵由于硅橡胶膜的渗透萃取即时分离,在很大程度上消除了产物香草醛的氧化损失和酶的产物抑制,原料丁香油转化率从0.038%提高到1.01%。高效液相色谱(HPLC)分析表明,香草醛在膜下游萃取液中的最高质量浓度达到了121.53 mg/L,远高于摇瓶实验的8.14mg/L,香草醛的峰面积比为70.08%。又在反应器中加入活性炭吸附剂,使转化率进一步提高到1.14%,膜下游萃取液最高质量浓度为140.27 mg/L,香草醛的峰面积比达到93.53%。The preparation of vanillin with clove oil as the substrate and soybean lipoxygenase - catalyst has been carried out in a silicon rubber membrane bioreactor （SRMBR）. Compared （SBLOX） as the bio with ferment in shaking flasks, the SRMBR system effectively prevented the product inhibition and oxidation of vanillin, owing to separation in situ through silicon rubber membrane. The bioconversion rate of clove oil was increased to 1.01% from 0. 038%. By high performance liquid chromatography （HPLC） analysis, the vanillin concentration of the receiving solution in membrane down - stream reached up to 121.53 mg/L, which was much higher than the 8.14 mg/L in the broth of the shaking flask ferment, and the peak area ratio of vaniUin in the receiving solution is 70.08%. By adding activated carbon as adsorbent into the broth, the bioconversion rate of clove oil was increased further to 1.14%, the vanillin concentration in the receiving solution reached 140.27 mg/L, and the peak area ratio of vanillin in the receiving solution has amounted to 93.53%.

关 键 词：香草醛 硅橡胶膜生物反应器 丁香油 酯氧化酶 渗透萃取 

分 类 号：TQ234[化学工程—有机化工]