慢性阻塞性肺病大鼠骨骼肌蛋白降解的变化  

Changes of skeletal muscle proteolysis in rats with chronic obstructive pulmonary disease

作　　者：杨天芸[1] 徐卫国[1] 罗勇[1] 韩锋锋[1] 陈迎春[1] 张悦[1] 万丹晶[2] 

机构地区：[1]上海交通大学医学院新华医院呼吸内科,上海200092 [2]复旦大学药学院放射药学教研组,上海200032

出　　处：《上海交通大学学报（医学版）》2008年第5期525-527,531,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委基金(05ZR14063)~~

摘　　要：目的探讨慢性阻塞性肺病(COPD)大鼠骨骼肌蛋白降解代谢变化的规律。方法利用熏香烟法制作COPD大鼠模型(COPD组),并设置对照组,每组20只。取伸趾长肌充氧离体孵育,采用高效液相-荧光法测量骨骼肌3-甲基组氨酸(3-MH)和酪氨酸(Tyr)的含量。结果孵育后,COPD组大鼠Tyr及3-MH的降解率与对照组比较,差异均有统计学意义(P<0.01,P<0.001)。结论COPD大鼠不仅全身骨骼肌总蛋白降解增加,作为机体蛋白质储存库重要组成部分的骨骼肌肌蛋白消耗更为明显。Objective To study the changes and rules of skeletal muscle proteolysis in rats with chronic obstructive pulmonary disease（COPD）. Methods Rat COPD model （ COPD group, n = 20） was established by passive cigarette smoking, and the control group（n = 20） was also established. After incubation in vivo with full oxygenation, contents of 3-methylhistidine（3-MH） and tyrosine（Tyr）in the extensor digitorium longus muscles were determined by high-performance liquid chromatography. Results After incubation, there were significant differences in the proteolytic rate of Tyr and 3-MH between COPD group and control group （P 〈 0.01, P 〈 0. 001 ）. Conclusion Total proteolytic rate and myofibrillar proteolytic rate in skeletal muscle are increased in rats with COPD.

关 键 词：慢性阻塞性肺病 骨骼肌 代谢 大鼠 

分 类 号：R563.9[医药卫生—呼吸系统]