经侧脑室注射IL-1ra对脑挫裂伤大鼠血浆S-100β蛋白和神经功能的影响  

Effect on serum S-100β protein contents and neural function in rats with cerebral contusion and laceration by injecting IL-lra into their lateral ventricle

作　　者：范学政[1] 姜军合[1] 翟安林[1] 李俊[1] 王帆[1] 

机构地区：[1]川北医学院第二附属医院神经医学中心,绵阳621000

出　　处：《中华神经医学杂志》2008年第5期471-473,478,共4页Chinese Journal of Neuromedicine

摘　　要：目的研究白细胞介素-1受体拮抗剂（IL-1ra）对大鼠脑挫裂伤后血浆S-100β蛋白含量变化和大鼠神经功能行为评分的影响，探讨IL—1ra脑保护作用的可能机制。方法雄性Wistar大鼠采用随机数字表法分为生理盐水组（n=30）及IL-1ra组（n=30），分别经侧脑室注射生理盐水、IL-Ira 5μL，并设立正常对照组m=5）。30min后按Feeney法将生理盐水组及IL-1ra组大鼠制作出脑挫裂伤模型，正常对照组不做任何处理。采用酶联免疫吸附试验（ELISA）测定脑挫裂伤后6h、12h、24h、2d、3d、7d各组大鼠血浆S-100β蛋白含量，Faden评分标准评定大鼠颅脑创伤后各时间点神经功能行为。结果（1）正常对照组大鼠血浆S-100β蛋白含量为（0．43＋0．04）μg／L，神经功能评分为35分。（2）伤后各时间点生理盐水组及IL-1ra组大鼠血清S-100β蛋白含量显著高于正常对照组（P〈0．05）。（3）IL-1ra组各时间点大鼠血清S-100β蛋白含量低于生理盐水组，且差异有统计学意义（P〈0．05）。（4）IL-1ra组伤后各时间点神经功能评分显著高于生理盐水组（P〈0．05）。结论（1）IL-1ra可降低脑挫裂伤大鼠血浆S-100β蛋白含量，提高大鼠神经功能评分。（2）IL—1ra对脑挫裂伤大鼠具有脑保护作用，可能与IL-1ra阻断IL-1β介导的损伤性脑细胞炎症反应有关。Objective To investigate the effect of IL-1ra on S-100β protein contents of serum and the behavior scales of neural function in rats with cerebral contusion and laceration by injecting IL-1ra into their later ventricle, and to explore the possible mechanism underlying the nerve protection of IL-1ra. Methods Male Wistar rats were randomly divided into 3 groups：normal saline group （NS group, NS 5μL injected into the left cerebral ventricle）, IL-1ra group （IL-1ra 5μg/5μL injected into the left cerebral ventricle）,and control group. After half an hour, cerebral contusion and laceration model was made according to Feeney＇s method in rats of NS group and IL-1ra group. The serum levels of S-100β protein in rats of each group were measured by ELISA at 6 h,12 h,24 h,2 d,3 d,7 d after hitting; and the behavior scales of neural function in all rats were evaluated based on Faden＇s method. Results （1）The level of serum S-100β protein was （0.43_＋0.04） μg/L, the score of neural function was 35 in rats of control group. （2）All the model rats＇ serum concentration of S-100β was higher than that of the control group markedly （P〈0.05） after injury. （3）S-100β concentration of rats in IL-1ra group was significantly lower than that of NS group at the each time point after injury （P〈0.05）,and the scores of neural function were higher than those of NS group （P〈0.05）. Conclusions （1）IL-1ra can decrease the level of serum S-100β protein and raise the behavior scores of neural function in rats. （2）IL-1ra has brain protective effect after cerebral injury, which may be related to the decrease of the inflammatory reaction of brain cell mediated by IL-1β.

关 键 词：脑挫裂伤 白细胞介素-1受体拮抗剂 白细胞介素-1β S-100Β蛋白 

分 类 号：R651.15[医药卫生—外科学]