大鼠鞘内阿米洛利及混合可乐定对神经病理性疼痛的作用  

Effects of Intrathecal Amiloride and Its Interaction with Clonidine on Treatment of Neuropathic Pain in Rats

作　　者：陈祥楠[1] 邢蔚[1] 曾维安[1] 欧阳汉栋[1] 李强[1] 王培宗[1] 刘先国[2] 

机构地区：[1]华南肿瘤重点实验室∥中山大学肿瘤防治中心 [2]中山大学中山医学院疼痛研究中心,广东广州510089

出　　处：《中山大学学报（医学科学版）》2008年第3期274-277,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30571794);国家自然科学基金(C03030301)

摘　　要：【目的】探讨大鼠鞘内阿米洛利及混合可乐定对神经病理性疼痛的治疗效能。【方法】102只SD大鼠,体质量(250～300g),随机分成17组(n=6):对照组、阿米洛利组、可乐定组、混合药物组、拮抗组等。鞘内置管后7d制作大鼠神经病理性疼痛模型(SNL模型)。建立疼痛模型前(基础值)、建立疼痛模型后1,3,5,7d及鞘内给药后测定大鼠机械性缩足反应阈值。计算各药物的半数有效剂量(ED50)及95%的可信区间。使用Isobolographic评价药物的相互作用。【结果】鞘内单独注射阿米洛利(12.5～100μg)或可乐定(0.5～10μg)可产生时间、剂量依赖性的抗神经病理性疼痛的作用(P<0.05);鞘内阿米洛利和可乐定混合,可以产生明显的协同效应;鞘内育亨宾(20μg)预处理可以拮抗鞘内阿米洛利、可乐定及阿米洛利混合可乐定的抗神经病理性疼痛的作用(P<0.05)。【结论】鞘内单独注射阿米洛利、可乐定可以产生明显的时间、剂量依赖性的抗神经病理性疼痛的作用;鞘内注射阿米洛利可以增强可乐定的抗神经病理性疼痛的作用。[Objective] To investigate the effects of intrathecal amiloride and its interaction with clonidine on the treatment of neuropathic pain in a rat model. [ Methods] One hundred and two male SD rats weighing 250-300 g were randomly divided into seventeen groups （n=6）： control group; amiloride group; clonidine group; amiloride ＋ clonidine group, and yohimbine group （amiloride ＋ clonidine ＋ yohimbine）. Seven days after intrathecal catheter placement, neuropathic pain model （spinal nerve ligation, SNL model） rats were developed. Paw withdrawal threshold were measured before SNL （baseline）, 1, 3, 5, and 7 days after SNL and after intrathecal drug treatment. Median effective dose （ED50） values and 95% confidence intervals were calculated. Isobolographic analysis of the amiloride and clonidine interaction was conducted. [ Result ] Intrathecal amiloride （12.5-100μg） or cloniding （0.5-10μg） alone produced a time- and dose-dependent treatment effect of neuropathic pain（P 〈 0.05）. The enhancing effects were found between intrathecal amiloride and clonidine. Intrathecal pretreatment with yohimbine （20μg） antagonized the treatment effects of amiloride, clonidine and the combination of amiloride and clonidine（P 〈 0.05）. [ Conclusion] Intathecal amiloride and clonidine alone produced a time- and dose-dependent treatment effects on neuropathic pain.The enhancing effects were found between intrathecal amiloride and clonidine.

关 键 词：神经病理性痛 阿米洛利 可乐定 协同作用 

分 类 号：R61[医药卫生—外科学]