脂肪组织中IRS-1相关的PI3K活性在多囊巢综合征胰岛素抵抗中的作用  

Insulin Receptor Substrate I-Associated Phosphatidylinositol 3-Kinase (PI3K) Activity in Adipose Tissue of PCOS

作　　者：王竹晨[1] 邱洁[1] 赖伟英[1] 丘惠娜[1] 胡钶[2] 顾熊飞[3] 杨冬梓[4] 邝健全[4] 

机构地区：[1]深圳市第二人民医院妇产科,广东深圳518035 [2]深圳市宝安区人民医院普外科,广东深圳518101 [3]中山大学中山医学院生物化学教研室,广东广州510080 [4]中山大学孙逸仙纪念医院妇产科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2008年第3期317-319,共3页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金博士科研启动基金(5301121);深圳市科技计划项目(200405034)

摘　　要：【目的】探讨多囊卵巢综合征(PCOS)脂肪组织胰岛素受体底物I相关的磷脂酰肌醇3激酶(IRS-1-Associcrted-PI3K)的活性在PCOS发病中的作用。【方法】PCOS患者和对照者根据体质量指数(BMI)分为PCOS肥胖组、PCOS非肥胖组、肥胖对照组和非肥胖对照组,各12例,采用免疫沉淀、薄层层析、放射自显影半定量检测脂肪组织IRS-1相关的PI3K活性。【结果】脂肪组织中IRS-1-Associated PI3K活性在PCOS肥胖组为55%±24%,较非肥胖对照组84%±16%明显降低(P<0.001);肥胖对照组为46%±22%,PCOS非肥胖组为71%±26%,均较非肥胖对照组明显降低(P<0.001和P<0.05),PCOS肥胖组和肥胖对照组之间以及PCOS肥胖组和PCOS非肥胖组之间的差异均无显著性意义(P>0.05)。【结论】PCOS肥胖组和PCOS非肥胖组脂肪组织IRS-1相关的PI3K活性均较非肥胖对照组明显减弱,可能抑制胰岛素受体后的信号传导,并与PCOS胰岛素抵抗的发生有关。[Objective] To study insulin receptor substrate Ⅰ-associated phosphatidylinositol 3-kinase （PI3K） activity of adipose tissue in polycystic ovary syndrome （PCOS） to approach the mechanism of insulin resistance （IR） in PCOS at the tissular and cellular level. [Method] Patients with PCOS and healthy controls were divided into the obese PCOS group, the non-obese PCOS group, the obese control group and the non-obese control group according to their body mass index （BMI） and with 25 cases each group. PI3K activity of adipose tissue were studied with immunoprecipitation, TLC, and phosphorimaging. [Result] PI3K activity of adipose tissue in the obese PCOS group （55% ± 24%, P 〈 0.001）, the obese control group （46% ± 22%, P 〈 0.001） and in the non obese PCOS group （71% ±26%, P 〈 0.05） were markedly lower than the non obese control group（84%± 16%）. The difference both between the obese PCOS group and the obese control group and the two PCOS groups were not significant （P 〉 0.05）. [Conclusion] PI3K activity of adipose tissue in the two PCOS groups both decrease markedly than the non obese control group. It might lead to the disorder of insulin-signaling transduction downstream of insulin receptor and concern with the IR.

关 键 词：多囊卵巢综合征 胰岛素抵抗 脂肪组织 胰岛素受体底物Ⅰ相关的P13K活性 

分 类 号：Q254[生物学—细胞生物学] R711.75[医药卫生—妇产科学]