胡黄连对缺血再灌注脑组织胶质源神经营养因子的影响  

EFFECTS OF PICRORHIZAE ON EXPRESSION OF GDNF AND GDNF mRNA AFTER CEREBRAL ISCHEMIA REPERFUSION

作　　者：辛萍[1] 杜芳[2] 

机构地区：[1]青岛大学医学院附属医院检验科,山东青岛266003 [2]青岛大学医学院附属医院脑血管疾病研究所,山东青岛266003

出　　处：《青岛大学医学院学报》2008年第2期114-116,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省卫生厅青年基金资助项目(2001CA2CBB2)

摘　　要：目的观察缺血再灌注大鼠脑内胶质源神经营养因子（GDNF）及GDNF mRNA的变化，探讨胡黄连对神经元的保护作用。方法成年健康雄性Wistar大鼠36只，应用线栓法制作脑缺血再灌注模型，用胡黄连甲醇提取物灌胃治疗后，免疫组织化学染色法观察皮质、纹状体GDNF蛋白表达，原位杂交方法观察其mRNA表达。结果模型组缺血侧皮质区于缺血再灌1d时GDNF蛋白、GDNF mRNA表达至高峰，与假手术组比较差异有显著性（F=14．168、40．189，q=12．565～16．847，P〈0．01），14d降至基础水平；纹状体区于缺血再灌3d时GDNF蛋白、GDNF mRNA表达至高峰，与假手术组比较有显著性差异（F=12．531、113．312，q=11．379～12．565，P〈0．01），14d降至基础水平；胡黄连组缺血再灌注14、21d时GDNF蛋白、GDNF mRNA表达与手术组及假手术组比较升高，有显著性差异（q=16．847～30．079，P〈0．01）。结论胡黄连对脑缺血再灌注损伤神经元有保护作用，其机制可能与上调脑内GDNF表达有关。Objective To observe the expression of glial derived neurotrophie factor （GDNF） and GDNF mRNA in cerebral tissue after reperfusion of focal cerebral isehemia, and to elucidate pierorhizae in the protection of neuron. Methods Isehemie reperfusion models were established with intraluminal thread method in 36 adult healthy male Wistar rats. The rats in model group were given intragastrie administration with pierorhizae, the expressions of GDNF and GDNF mRNA in cortex and striatum were determined respectively by immunohistoehemieal and in situ hybridization assay. Results In model group, the GDNF and GDNF mRNA positive neurons increased from 6 h, peaked at 1 d in cortex and peaked at 3 d in striatum. Compared with operation group and sham-operation group, the expressions of GDNF and GDNF mRNA increased insignificantly in treatment group. Conclusion Pierorhizae could protect the neurons against cerebral hypoxia-isehemie damage by increasing the expressions of GDNF and GDNF mRNA.

关 键 词：胡黄连 脑缺血再灌注 胶质源性神经营养因子 大鼠 Wistar 

分 类 号：R285[医药卫生—中药学]