染料亲和膜的制备及其吸附白蛋白的性能研究  

Preparation of Dye Affinity-membrane and Its Adsorption Properties for Bovine Serum Albumin

作　　者：姜海容[1] 

机构地区：[1]厦门大学化学化工学院化学实验教学中心,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第1期71-74,共4页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金(C0510005)资助

摘　　要：以尼龙66膜为基膜,采用甲醛活化法偶联壳聚糖制备尼龙-壳聚糖复合膜,再固载染料Cibacron Blue F3GA作为配位基,制备亲和膜.膜上壳聚糖和Cibacron Blue F3GA的含量分别为112.4和123.3 mg/g尼龙膜.通过静态和动态实验测定了牛血清白蛋白在膜上的吸附性能,结果表明:牛血清白蛋白在膜上的吸附平衡符合Langmuir方程;在蛋白溶液的pH值为4.7和较低的离子强度下对牛血清白蛋白具有较高的吸附量;在动态操作条件下容易达到吸附平衡.Using Cibacron Blue F3GA as ligand,the affinity nylon-chitosan composite membrane was prepared and its adsorption characteristics for bovine serum albumin（BSA） were studied.Microporous nylon membranes were activated by formaldehyde and subsequently bound with chitosan to amplify reactive groups.Then Cibacron Blue F3GA was immobilized onto the chitosan-coated nylon membranes.The contents of chitosan and Cibacron Blue F3GA of affinity membranes were 112.4 and 123.3 mg/g nylon membrane,respectively.The obtained Cibacron Blue F3GA-chitosan affinity membranes were used to adsorb BSA from the BSA solution.The adsorption mechanism of BSA and the effects of pH value and ionic strength on adsorption were investigated by batch experiments.The results showed that the adsorption capacity increased with increasing BSA initial concentration while decreased with increasing the NaCl concentration and the adsorption isotherm fitted the Langmuir model well.The Cibacron Blue F3GA-chitosan affinity membranes exhibited the high adsorption capacity for BSA.

关 键 词：亲和膜 壳聚糖 白蛋白 吸附 

分 类 号：Q647.31[生物学—生物物理学] TQ424.3[化学工程]