牡蛎低分子活性肽BPO-L对人肺腺癌A549细胞周期和相关癌基因、抑癌基因表达的调控作用  

The Regulation Effects of Bioactive Peptides of Oyster(BPO-L) on the Cell Cycle and Gene Expression of Human Lung Adenocarcinoma A549 Cells

作　　者：李祺福[1] 黄大川[1] 石松林[1] 梁盈[1] 李筱泉[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第1期104-110,共7页Journal of Xiamen University：Natural Science

基　　金：福建省科技计划重点项目(2003N0052)资助

摘　　要：采用酸抽提、凝胶柱层析等方法,从僧帽牡蛎体内分离提取到牡蛎低分子活性多肽组分BPO-L,以HMBA处理组为平行对照,流式细胞仪检测细胞周期变化及以免疫细胞化学方法检测相关癌基因、抑癌基因表达变化.研究BPO-L对人肺腺癌A549细胞分化的生物学效应,探索其对肺癌细胞的作用机理.实验结果显示,BPO-L能有效抑制A549细胞增殖活动,促使细胞阻滞于G0/G1期.在此过程中,A549细胞c-myc,MTp53等癌基因蛋白表达减弱,p21WAF1/CIP1和Rb等抑癌基因蛋白表达活性的增强.本研究证实BPO-L对肺癌细胞具有显著的诱导分化作用.其诱导癌细胞分化机理与其调节和干预c-myc、MTp53等癌基因与p21WAF1/CIP1和Rb等抑癌基因的表达有关.To investigate the effects and mechanism of bioactive peptides of oyster（BPO-L）on the differentiation of human lung adenocarcinoma A549 cells,BPO-L,isolated from Saccostrea cucullata by acid extraction and gel chromatography,was used to treat the A549 cells.HMBA was used as control.Cell cycle was detected by flow cytometer,some oncogenes and tumor repressive genes were detected by immunocytochemistry analysis.The results showed that BPO-L could effectively inhibit the proliferation of A549 cells,and arrest the cells in G0/G1 phase.BPO-L,HMBA and their combination could up-regulate the expression of gene p21WAF1/CIP1and Rb,and down-regulate gene c-myc and mt p53.It could be concluded that BPO-L could effectively induce A549 cells into differentiation,and the induced-differentiation effects related with the changes of oncogenes and tumor repressive genes.

关 键 词：人肺腺癌A549细胞 牡蛎低分子活性肽 细胞周期 癌基因与抑癌基因 诱导分化 

分 类 号：Q254[生物学—细胞生物学]