细菌性眼内炎的病原分子诊断研究  

Application of molecular method in identification of bacterial endophthalmitis

作　　者：吴薇[1] 鲁辛辛[1] 黄艳飞[1] 

机构地区：[1]首都医科大学附属北京同仁医院检验科,100730

出　　处：《中华医学杂志》2008年第20期1429-1432,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30672006）

摘　　要：目的以16S rRNA部分基因直接测序为基础分析细菌性眼内炎中的病原菌，建立眼内标本病原菌非培养检测方法。方法收集50例患者房水或玻璃体液标本，提取细菌DNA，16S rRNA基因通用引物PCR，扩增产物直接测序，通过核酸序列比对微生物种属鉴定，同时与传统方法进行比较。结果50例标本中，直接涂片阳性22例（44％），细菌培养阳性13例（26％），PCR检测阳性28例（56％），成功测序22例，序列鉴定率为44％。16S rRNA基因序列鉴定与培养鉴定结果不完全吻合。结论分子诊断对明确细菌性眼内炎感染有指导意义。Objective To search for a non-culture method to improve the efficiency of etiological diagnosis of infective endophthalmitis and other infectious diseases. Methods Fifty clinical specimens of infective endophthalmitis were collected. DNA of bacterium was extracted directly from the aqueous fluid or vitreous fluid and then amplified with universal primers. The PCR products were sequenced subsequently. Results Twenty-two （44%） bacterial smears and 13 （26%）cultured specimens were positive. PCR positivity was seen in 28 of the 50 cases（56% ） and 22 specimens （44%）were sequenced successfully. The results of 16s rRNA gene sequence analysis did not completely conform to the results obtained from culturedependent approach. Conclusion Molecular identification method is a rapid and efficient approach for the diagnosis of bacterial infective endophthalmitis.

关 键 词：细菌 RNA 序列分析 

分 类 号：R686[医药卫生—骨科学]