甘肃中部梨种质资源的AFLP分析  

Assessment of pear germplasm from the middle area of Gansu province using amplified fragment length polymorphism markers

作　　者：蔡丹英 范太伟[2] 滕元文[1] 赵长增[2] 陈佰鸿[2] 王发林[3] 

机构地区：[1]浙江大学园艺系,农业部园艺植物生长发育与生物技术重点开放实验室,杭州310029 [2]甘肃农业大学农学院园艺系,兰州730070 [3]甘肃省农业科学院果树科学研究所,兰州730070

出　　处：《果树学报》2008年第3期298-304,共7页Journal of Fruit Science

基　　金：国家自然科学基金(3037098)

摘　　要：采用AFLP分子标记技术对甘肃中部梨种质资源的遗传多样性进行分析。结果表明,6对AFLP引物在40份甘肃梨种质中共扩增出472条带,其中多态性带为404条,多态率高达86%,显示了甘肃中部梨资源丰富的遗传多样性。任何一对引物可以鉴别所有40份资源,显示了很高的鉴别能力。采用UPGMA聚类分析法构建的系统树在相似系数为0.72时将40份种质分为7个组:西洋梨、新疆梨、白梨(砂梨)、木梨、褐梨组和2个秋子梨组。所有白梨品种与唯一的砂梨品种黄花梨聚为一个大组。形态学上归属不明的品种分别聚类到西洋梨、白梨、木梨和秋子梨组中。研究表明基于AFLP标记的梨资源分类体系可以反映甘肃地方梨品种和类型间的遗传多样性和亲缘关系。Genetic diversity and genetic relationship of 40 accessions of pear （Pyrus L.） germplasm native to the middle area of Gansu province in China were analyzed using amplified fragment length polymorphism （AFLP）marker. 6 primer combinations generated 472 scored bands, among which 404 （86%） were polymorphic, which showed the abundant genetic diversity of these pear gerrmplasm. Based on the UPGMA cluster analysis, 40 pear accessions could be obviously divided into 7 major groups at the level of the coefficient of 0.72： P. comrnunis L, P. sinkiangensis Yu, P. pyrifolia Nakai var. sinensis Y. Teng et K. Tanabe （Chinese white pears）, P. xerophila Yu, P. phaeocarpa Rehd. and two P. ussuriensis Maxim groups. All the Chinese white pears were clustered into one group with Huanghuali, which belonged to P. pyrifolla. Unidentified pear euhivars or types were clustered with P. ussuriensis, P. communis, P. xerophila and Chinese white pears, respectively. The research indicated that the classification system based on AFLP could reflect the genetic diversity and relationship of Pyrus species and cultivars from Gansu province.

关 键 词：梨属 甘肃 AFLP 遗传多样性 亲缘关系 

分 类 号：S661.2[农业科学—果树学]