梨矮化基因pcDw的SSR标记定位  

Location of a pear dwarf gene pcDw by SSR marker

作　　者：田义轲[1] 王彩虹[1] 贾彦利[1] 王亮[1] 戴洪义[1] 

机构地区：[1]青岛农业大学园艺学院,山东青岛266109

出　　处：《果树学报》2008年第3期404-407,共4页Journal of Fruit Science

基　　金：国家"863"计划(2006AA100108-3-5);山东省自然基金(Y2003D01);青岛市科技攻关(05-2-NS-18)

摘　　要：以矮化梨(Pyrus communis L.)与茌梨(P.bretschneideri Rehd.)的F1杂交分离群体共110个单株(3a生,矮化型和正常型各55株)为试材,对来自西洋梨的矮化型突变基因pcDw进行了SSR分子标记研究。用分离群体分组分析法(Bulked Segregant Analysis,BSA),通过对源自梨、苹果和桃基因组的共40对SSR(Simple Sequence Repeat)引物的筛选,获得了一个与pcDw基因连锁距离为9.3cM的SSR标记KA14210,由此将该基因定位到了梨品种Barlett遗传图谱的第16连锁群上。The F1 segregation population, total of 110 （3-year-old） seedlings derived from the cross Aihuali （Pyrus communis L.） ×Chili （P. bretschneideri Rehd.） was tested for identifying SSR molecular markers linked to a pear dwarf gene pcDw, which produced by a mutation of P. communis. Altogether, 40 pairs of SSR primers originating from pear, apple and peach genome were screened by bulked segregant analysis （BSA）. Finally, a SSR marker, KA14210, which linked topcDw gene at a distance of 9.3 cM, was acquired. By this marker, pcDw gene was located on LG16 of the genetic map of pear variety Barlett.

关 键 词：梨 矮化性状 pcDw基因 SSR标记 图谱定位 

分 类 号：S661.2[农业科学—果树学]