香蕉凝集素基因双启动子表达  

Activity characterization of a banana lectin gene double promoter

作　　者：张建斌[1] 赵静[1] 黎耀平[1] 金志强[1] 徐碧玉[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《果树学报》2008年第3期422-425,共4页Journal of Fruit Science

基　　金：国家自然科学基金项目(30370987);江苏省应用基础项目(BJ200018)

摘　　要：为了获得香蕉果实特异表达的强启动子,利用PCR方法将2个凝集素(BanLec)基因启动子串联在一起,置换植物表达载体pBI121上的35S启动子,构建BanLec基因双启动子携带gus植物表达载体pBIL3,用基因枪转化香蕉叶片、根系和果实薄片,瞬时表达结果显示BanLec基因串连的双启动子依然表现为果实特异性表达,而且表达量明显高于BanLec基因单启动子及35S启动子,证明该串连的双启动子是一个在香蕉果实中特异表达且表达量较高的强启动子。In order to acquire an enhanced promoter expressed specifically in banana fruit, a double BanLec gene promoter were constructed by PCR method. The 35S promoter in the expression vector pBI121 was substituted with the double promoter to construct a new plant expressed vector pBIL3, which was then ligated with gus gene. Banana leaves, roots and fruit sections were transformed with this vector. The result indicated that this double promoter was fruit-specific and its expression was stronger than single BanLec gene promoter and 35S promoter.

关 键 词：香蕉 凝集素基因 双启动子 果实特异表达 

分 类 号：S668.4[农业科学—果树学]