电针对SAMP8小鼠海马神经元线粒体超微结构的影响  

Influence of Electroacupuncture on the Mitochondrial Ultrastructure in the Hippocampal Neurons of SAMP8 Mice

作　　者：曾芳[1] 何宇恒[2] 彭静[1] 唐勇[1] 尹海燕[1] 余曙光[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川610075 [2]西藏藏医学院基础部,拉萨850000

出　　处：《上海针灸杂志》2008年第5期41-43,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然基金(30472235);四川省教育厅自然科学青年基金(2006B023)

摘　　要：目的观察电针对SAMP8小鼠海马神经元线粒体超微结构及相关凋亡蛋白的影响,从保护线粒体、抑制细胞凋亡的角度探讨电针治疗阿尔茨海默病(AD)的部分作用机制。方法以SAMP8小鼠为研究对象,依据"补肾通督,醒神益智"的治疗原则,电针"百会"、"涌泉",每日1次,共治疗21天。用Morris水迷宫测试小鼠学习记忆能力的变化,评价电针对AD的治疗效应。用透射电镜观察海马神经元线粒体的超微结构。结果模型组与空白组相比较,模型组小鼠平均逃避潜伏期明显延长,原平台象限停留时间明显缩短,海马神经元线粒体超微结构不完整,线粒体面密度和体密度减少;模针组与模型组相比较,模针组小鼠治疗后平均逃避潜伏期缩短,原平台象限停留时间延长,学习记忆能力有所提高,海马神经元线粒体超微结构基本正常,线粒体面密度和体密度增加。结论电针对AD治疗效应的发挥可能与减轻线粒体超微结构损伤,改善线粒体功能,进而改善能量代谢和脑功能有关。Objective To investigate the influence of electroacupuncture on the mitochondrial ultrastructure and related apoptosis protein in the hippocampal neurons of SAMP8 mice and explore partial mechanism of the therapeutic effect of electroacupuncture on AD. Methods SAMP8 mice were selected as subjects. According to the therapeutic principle of reinforcing the kidney, unblocking the Du Meridian, activating the mind and improving intelligence, Baihui （GV 20） and Yongquan （KI 1 ） were given electroacupuncture once daily, 20 days in total. The Morris water maze was used to test mouse learning and memory abilities and assess the therapeutic effect of electroacupuncture on AD. The mitochondrial ultrastructure in the hippocampal neurons was observed under a transmission electron microscope. Results Average escape latency significantly lengthened, the plateau phase significantly shortened, the mito- chondrial uhrastructure in the hippocampal neurons was incomplete and mitochondrial surface density and body density decreased in the model group compared with the blank group. Average escape latency shortened, the plateau phase lengthened, learning and memory abilities somewhat improved, the mitochondrial uhrastructure in the hippocampal neurons was basically normal and mitochondrial surface density and body density increased in the model-acupuncture group after treatment compared with the model group. Condusion The therapeutic effect of electroacupuncture on AD may be related to reducing injury to mitochondrial ultrastructure and improving mitochondrial function and thereby energy metabolism and brain function.

关 键 词：电针 阿尔茨海默病 线粒体超微结构 

分 类 号：R2-03[医药卫生—中医学]