羟喜树碱在导电聚合物膜修饰电极上的电催化性能及其电化学分析方法研究  

Electrocatalytic Performance of Hydroxycamptothein at the Electropolymer Film Modified Electrode and Its Electroanalytical Application

作　　者：刘爱林[1] 张少波[1] 李丽萍[1] 吴潇霎[1] 曹扬远[1] 林新华[1] 

机构地区：[1]福建医科大学药学院药物分析系,福建福州350004

出　　处：《分析测试学报》2008年第5期484-487,共4页Journal of Instrumental Analysis

基　　金：国家863计划资助项目(2006AA02Z4Z1);国家自然科学基金资助项目(20675015);福建省科技重点基金资助项目(200610016);福建省青年人才基金资助项目(2006F3058)

摘　　要：采用循环伏安法对羟喜树碱在4-(2-吡啶偶氮)间苯二酚(PAR)导电聚合膜修饰电极上的电催化性能及其电化学分析方法进行了研究。磷酸盐缓冲液中(pH 3.0),在-0.2^+0.4 V范围内羟喜树碱在PAR膜修饰电极表面受吸附控制,发生准可逆单电子转移电极反应过程,电子转移系数α=0.38;首次提出了以差示脉冲伏安法建立检测羟喜树碱含量的新方法。在聚合时间为40圈(100 mV/s),富集电位+1.0 V,富集时间240 s条件下,利用差示脉冲伏安法测得其氧化峰电流Ip与浓度分别在0.01~1.0μmol/L和1.0~4.0μmol/L范围内呈良好的线性关系,检出限为1.0 nmol/L。The electrocatalytic performance of hydroxycamptothein （ HCPT ） at the eleetropolymer 4- （2-pyridylazo） -resorcinol （PAR） film modified glassy carbon electrode （ GCE ） and its electroanalytical application were studied by cyclic voltammetry. The results showed that HCPT exhibited a very high electrocatalytic activity at the PAR modified electrode. In the range of-0. 2 - ＋ 0. 4 V and in a phosphate buffer solution（ pH 3.0） , a well-defined redox peak of HCPT was observed. It was found that the reaction process of HCPT at the surface of PAR film modified electrode was an adsorption-controlled and a quasi-reversible single electron charge transfer process. The calculated charge transfer coefficient （α） was 0. 38. Based on these, a novel method for the determination of HCPT by differential pulse voltammetry was proposed. The linear ranges of the regression curves for HCPT concentration were 0. 01 - 1.0 μmol/L and 1.0 -4.0 μmol/L, respectively. The detection limit was 1.0 nmol/L.

关 键 词：4-(2-吡啶偶氮)间苯二酚(PAR)修饰电极 导电聚合膜修饰电极 羟喜树碱 循环伏安法 生物碱类化合物 

分 类 号：O657.15[理学—分析化学] Q946.88[理学—化学]