高脂血症小鼠心脏和肺组织中eNOS及HO-1的表达  

Expression of eNOS and HO-1 in heart and lung tissues of hyperlipidemia mice

作　　者：江一峰[1] 曹晓娥 王丽[1] 赵凤娣[1] 殷莲华[1] 

机构地区：[1]复旦大学上海医学院生理学与病理生理学系,上海200032 [2]上海职工医学院生理学系,上海200237

出　　处：《复旦学报（医学版）》2008年第3期342-347,共6页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目(30470689)

摘　　要：目的观察高脂饲料喂养的小鼠心脏和肺组织中内皮型一氧化氮合酶(endothelial nitric-oxide syn-thase,eNOS)及血红素氧合酶-1(heme oxygenase-1,HO-1)的含量变化,探讨高脂血症对eNOS和HO-1表达的影响。方法高脂饮食组(n=8)和普通饮食组(n=8)C57BL/6小鼠分别经高脂饲料和普通饲料喂养18周后,测血清总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、高密度脂蛋白(high density lipopro-tein,HDL)和低密度脂蛋白(low density lipoprotein,LDL)含量,采用Real-ti me PCR检测心脏和肺组织中eNOS及HO-1的mRNA水平,以免疫组化方法检测其蛋白表达。结果高脂饮食组TC、TG和LDL明显高于普通饮食组,HDL则低于普通饮食组;高脂饮食组心脏和肺组织中eNOS的mRNA和蛋白水平低于普通饮食组,HO-1的mRNA和蛋白水平高于普通饮食组。结论高脂饲料喂养能使小鼠形成高脂血症,并引起心脏和肺组织中eNOS的表达降低,同时HO-1的表达升高。Objective To explore the effects of hyperlipidemia in high-fat diet mice on expression of endothelial nitric-oxide synthase （eNOS） and heme oxygenase-1 （ HO-1 ） in heart and lung tissues. Methods C57BL/6 mice were fed a high-fat diet as high-fat diet group （n = 8, high-fat diet group） and were fed a normal diet as control （n= 8, normal diet group）. Eighteen weeks later, serum lipid profiles including total cholesterol （TC）, triglyceride （TG）, high density lipoprotein （HDL） and low density lipoprotein （LDL） were detected, eNOS and HO-1 mRNA expression in heart and lung tissues were determined by Real-time PCR, as well as protein expression by immunohistochemistry. Results Levels of serum TC, TG and LDL significantly increased in high-fat diet group, while HDL decreased, mRNA and protein expression of eNOS in heart and lung tissues reduced in high-fat diet group, while HO-1 increased. Conclusions High-fat diet may lead to hyperlipidemia in mice, which may down-regulate eNOS expression and up-regulate HO-1 expression in heart and lung tissues.

关 键 词：高脂血症 内皮型一氧化氮合酶 一氧化氮 血红素氧合酶-1 一氧化碳 

分 类 号：R589.2[医药卫生—内分泌]