缓释bFGF微球体外降解和释药性质研究  

The Experiment of Biodegradation and Drug Release Properties of bFGF Controlled Release Microspheres in vitro

作　　者：段宏[1] 裴福兴[1] 胡隽宇[1] 屠重棋[1] 刘钰[2] 樊瑜波[3] 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]中国科学院成都有机化学研究所,成都610041 [3]北京航空航天大学生物工程系

出　　处：《中国生物医学工程学报》2008年第3期431-437,467,共8页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10402025)

摘　　要：通过体外降解释药实验，初步探索bFGF微球在体外的降解释药特点。以PBS液为体外降解和释药介质，初步研究bFGF微球体外降解和释药特点。bFGF微球与空白微球体外降解和质量丧失趋势一致，各个指标之间差异无显著性。约在10d时PLGA的重均分子相对质量下降一半，14—18d时微球质量下降一半，说明微球质量丧失滞后于重均分子相对质量下降。突释期内微球体外释放度仅为19．26％，21d后体外累积释放度高达85．46％，释出bFGF浓度稳定，其平均浓度为（72．47±6．26）ng·mL^-1，微球的体外释药规律符合Higuichi方程（r=0．9978）。本实验bFGF微球与空白微球降解和吸收趋势一致。聚合物的质量和形态结构变化相对滞后于重均分子相对质量的下降，降解可能主要发生在分子链断裂。The biodegradation and drug release characteristic of bFGF microspheres （Ms） were investigated. With PBS solution used as medium, the biodegradation characteristic bFGF Ms was investigated at a primary attempt, and the drug release characteristic of bFGF Ms was also studied. The trend of in vitro molecular （Mw） loss and mass loss of PLGA of bFGF Ms was consistent with that of empty ones, showing that all indexes had no significant difference. The mean Mw of PLGA decreased by 50% about 10 days, while the mass of Ms diminished by half until 14 - 18 days later, all of which could account for the fact that the mass loss of the Ms was not at the same level of that of the molecular weight, the latter was faster. The ratio of drug release in vitro amounted to only 19.26% in the burst release phase, but rose to 85.46% accumulatively after 21 days. The concentration of bFGF released from Ms was stable with the average concentration of （72.47 ± 6.26） ng· mL^-1 . The law of in vitro drug release of bFGF Ms corresponded with Higuiehi equation（ r = 0.9978） . The trend of in vitro moleeular（Mw） loss and mass loss of PLGA of bFGF Ms were similar with that of empty ones. Changes of the mass loss and the shape were comparatively slower than that of the molecular weight loss. It might come to the conclusion that biodegradation may have taken place in molecular chain.

关 键 词：碱性成纤维细胞生长因子 聚乳酸-聚羟基乙酸共聚物 微球 缓释 降解 

分 类 号：R318.08[医药卫生—生物医学工程]