棕囊藻渤海株核糖体18SrDNA和ITS基因结构序列分析  

Structure and Sequence Analysis of 18s rDNA and ITS Gene of Phaeocystis Isolate From the Bohai Sea

作　　者：曲凌云[1] 吕颂辉[2] 高春蕾[1] 李艳[1] 孙萍[1] 孙修勤[1] 

机构地区：[1]国家海洋局第一海洋研究所 [2]暨南大学赤潮与水环境研究中心,广东广州510632

出　　处：《海洋科学进展》2008年第2期200-206,共7页Advances in Marine Science

基　　金：科技部公益项目--保障食品安全的贝毒早期监测预警研究(2005DIB2J116);国家科技基础平台工作项目--近海海洋微生物资源的标准化整理整合与共享(2004DKA30640);所长基金--渤海棕囊藻赤潮的形成与危害机理研究

摘　　要：对分离自渤海赤潮发生区的1株棕囊藻进行核糖体18S rDNA及ITS序列克隆测定,获得了长度为1 648bp的18S rDNA序列和长度为890 bp的ITS序列。对获得的基因序列进行同源性分析,结果表明,该棕囊藻的核糖体18S rDNA及ITS序列均与NCBI数据库中登录的球形棕囊藻的相应序列同源性最高;从GenBank中获取不同地理来源的19种棕囊藻的18S rDNA序列及6种棕囊藻的ITS序列,分别以棕囊藻核糖体18S rDNA和ITS为对象构建了棕囊藻属的系统发育树,从分子生物学角度确定了棕囊藻渤海株为球形棕囊藻(Phaeocystis globosa)。The sequences of 18S rDNA and ITS gene of Phaeocystis isolate from the Bohai Sea were cloned and analyzed, and a 18S rDNA gene sequence of 1648 bp in length and an ITS gene sequence of 890 bp in length were obtained. It is shown from the homologous analysis results that the obtained 18S rDNA and ITS gene sequences are highly homologous to the relevant sequences of P. globosa in GenBank. The 18S rDNA sequences of 19 Phaeocystis species and ITS sequences of 6 Phaeocystis species were retrieved from GenBank, and the phylogenetic tree of Phaeocystis is then constructed by using these sequences and neigh-bor-joining method. It is shown from molecular biological analysis results that the Phaeocystis isolate from the Bohai Sea is identified as Phaeocystis globosa.

关 键 词：棕囊藻 18S RDNA基因 ITS基因 系统发育分析 

分 类 号：Q786[生物学—分子生物学]