复方木鸡冲剂抑制HepG2细胞增殖及诱导分化的作用  

Study on inhibition proliferation and differentiation of human hepatoma cell line （HepG2） induced by Fufang Muji Chongji

作　　者：郝晓娜[1] 郭莲英[2] 邢嵘[2] 

机构地区：[1]蚌埠医学院检验系核医学教研室,安徽蚌埠233030 [2]大连医科大学肿瘤生物治疗研究所辽宁,大连116044

出　　处：《中国中医药杂志》2008年第5期3-7,共5页

摘　　要：目的：探究复方木鸡冲剂在体外对人肝癌细胞株HepG2的抗增殖作用及其机制。方法：光镜观察细胞形态的变化。MTT法检测木鸡冲剂对肿瘤细胞增殖的抑制作用。流式细胞仪分析细胞凋亡的情况。全自动生化分析仪检测培养上清液中GGT、ALP浓度的变化；免疫细胞化学法检测细胞周期依赖性蛋白激酶抑制因子P21^WAFI的表达。结果：复方木鸡冲剂对HepG2有明显的抑制生长作用．细胞凋亡率明显增高，培养上清中ALP浓度升高，AFP浓度降低,P21^WAFI表达呈强阳性，而GGT没有明显变化。结论：复方木鸡冲剂明显抑制HepG2细胞的生长，其抗肿瘤的机制与诱导肿瘤细胞凋亡和促分化有关。Object To study the inhibition effect and mechanism of Muji Chongji on hepatocarcinoma cell line HepG2. Methods MTT assay was adopted to describe the proliferation of carcinoma cells. Apoptosis induced by Muji Chongji was investigated by applying light microscopy and flow cytometry （FCM）, The contents of γ-glutamyl transpeptidase （γ-GT） and alkaline phosphatase （ALP） in the supernatant were determined by biochemistry techniques, α-fetoprotein （AFP） was detected by radioimmunoassay. Immunohistochemical staining was performed to determine the protein of p21^WAFI. Results： Our results showed Muji Chongji reduced HepG2 cell viability obviously（p〈0.01）; changes of apoptosis in morphology of HepG2 treated with Muji Chongji was appeared and apoptotic cells rate increased （p〈0. 05）; significantly decreased AFP value of HepG2 secretion and increased ALP value. Our study also showed that Muji Chongji induced up-regulation of p21^WAFI protein. Conclusion Fufang muji Chongji can inhibit the proliferation of HepG2 cells and induce apoptosis in HepG2 cells. The mechanism of anti-tumor activities of Fufang muji Chongji is that induce differentiation in HepG2 cells and there is significant correlation with cell cycle inhibitor p21^WAFI.

关 键 词：复方木鸡冲剂 肝细胞癌 凋亡 分化 

分 类 号：R286[医药卫生—中药学]