二甲亚砜对染毒支气管上皮细胞的保护作用分析  

The protection of DMSO to cytotoxic of human bronchial epithelial cell caused by uranium mine dust

作　　者：李世珍[1] 黄波[1] 黄锐[1] 贺性鹏[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《中国医学工程》2008年第1期26-28,31,共4页China Medical Engineering

基　　金：国家自然科学基金(No.30670634)

摘　　要：目的在观察铀矿粉对体外培养的支气管上皮细胞的毒性作用同时,加入二甲亚砜,以了解其在支气管上皮细胞受铀矿粉染毒时的可能保护机制。方法将不同浓度的铀矿粉与体外培养的支气管上皮细胞进行长时间接触,分为对照组、染毒组和二甲亚砜(DMSO)保护组,通过血清抗性实验等,观察各组间细胞受损的情况。结果随着铀矿粉浓度的增高,支气管上皮细胞受损明显加重,加入二甲亚砜的支气管上皮细胞受损情况好于未加入者。DMSO对铀矿尘作用于BEAS-2B细胞后,可引起细胞DNA断裂,造成细胞拖尾率、彗星尾部DNA含量、尾长、尾部面积、尾距增加具有较好的抑制作用。结论铀矿粉对支气管上皮细胞有明显的恶性转化作用,可能是铀矿粉对肺损伤的主要机制,二甲亚砜具有一定的保护作用。[Objective] The aim of this study was to explore the malignant transformation effect on cytotoxic of human bronchial epithelial cell （BEAS-2B） induced by uranium mine dust, further investigate the cause and mechanism of human lung cancer. All of these is to be researched the protection and mechanism of DMSO to cytotoxic, genotoxic, clastogenic and mutagenic which caused by uranium mine dust. [Methods] The immortalized human bronchial epithelial cells BEAS-2B were treated with uranium mine dust at the concentrations of d iffent dose, including a negative control group and the group protected by DMSO. The characteristics of the cellular biology and the malignant transformation phenotype of cells were identified through observing serum resistance, etc. [Results] The mutation frequency of BEAS-2B cells were significantly increased by uranium mine dust and the damaged degree will aggravate with high concentration. Uranium mine dust induced cell damage will reduced effectively by protected of DMSO. Uranium mine dust induced cell DNA single breaks,tail area, tail DNA%, tail length, tail moment, olive tail moment increased significantly and these increments could be inhibited effectively by DMSO. [ Conclusons] Uranium mine dust could induce malignant transformation of BEAS-2B cells. DMSO can prevent damage of Uravdum mine dust.

关 键 词：铀矿粉 人支气管上皮细胞 二甲亚砜 

分 类 号：R142.3[医药卫生—公共卫生与预防医学]