大孔吸附树脂对红车轴草中总异黄酮的提取纯化工艺研究  

Study on Extraction and Purification of Total Isoflavones from Trifolium Pratense L. by Macroporous Obsorption Resin

作　　者：奚静芳[1] 周健[1] 李亚尊[1] 

机构地区：[1]上海市中药研究所,上海201203

出　　处：《中国现代中药》2008年第5期21-23,31,共4页Modern Chinese Medicine

摘　　要：目的:筛选红车轴草中异黄酮的最佳提取条件,研究大孔树脂富集、纯化总异黄酮的工艺条件与参数。方法:以总异黄酮的含量和重量为指标,采用正交试验筛选红车轴草的最佳提取方法和吸附条件。结果:红车轴草的最佳提取工艺为20倍量的70%乙醇,提取2次,每次1.5h,洗脱条件为以4倍量柱体的水和30%乙醇洗去杂质,再以4倍量的90%乙醇洗脱,收集总异黄酮的含量和得率最高;最佳上柱条件为药液浓度1∶4,径高比1∶20,药液洗脱流速20mL·min-1。结论:采用此法可较好地富集、纯化红车轴草中的总异黄酮。Objective： To screen the best method of extraction of total isoflavones from Trifolium pretense L.. To study the technological parameters of the purification process of total isoflavones from Trifolium pretense L.. Methods： Use the content and weight of total isoflavones as maker to screen the best method of extraction and obsorption parameters by orthogonal design. Results：The best method of extraction were extracting the Trifolium pretense L two times with 20BV 70% ethanol and the extraction time is 1.5 hour. The best desorption condition is washing the resin volume with 4BV 90% ethanol. The result showed that extract solution at a concentration 1：4, ratio of column diameter to column height 1：20, flow rate of 20mL·min^-1 as the optimal condition. Conclusion： The purification of total isoflavones from Trifolium pratense L is reliable and efficacious.

关 键 词：红车轴草 异黄酮 大孔吸附树脂 提取纯化 

分 类 号：R286[医药卫生—中药学] R284.2[医药卫生—中医学]