固定化反胶团漆酶及其在修复土壤DDT污染中的应用  

Immobilization of laccase in reverse micelles and its use in remediation of DDT-contaminated soil

作　　者：赵月春[1] 付蓉[1] 莫测辉[2] 易筱筠[3] 

机构地区：[1]华南农业大学应用化学系,广东广州510640 [2]暨南大学环境工程系,广东广州510632 [3]华南理工大学环境科学与技术系,广东广州510640

出　　处：《生态环境》2008年第2期606-610,共5页Ecology and Environmnet

基　　金：广东省自然科学基金项目(021016;036716);广东省科技计划项目(2002C31603;2003C34505);广州市科技计划项目(2007Z3-E0471)

摘　　要：采用吸附法将反胶团漆酶吸附在表面改性后的硅藻土上,制备固定化反胶团漆酶。探讨了反胶团漆酶固定化的影响因素及其部分酶学特性,并对其在修复土壤DDT污染中的应用进行了研究。反胶团漆酶固定化的最佳温度是35℃,载体硅藻土的改性剂Tween-80的加入量为硅藻土质量的15%。固定化反胶团漆酶的最适作用温度为35℃,最适作用pH为3.5～5.0;与游离漆酶相比,固定化反胶团漆酶的热稳定性和酸碱稳定性都显著提高。采用游离漆酶和固定化反胶团漆酶修复DDT污染土壤,游离漆酶处理中DDT总量(DDTs)的降解率为50.53%,而固定化反胶团漆酶处理中DDTs的降解率高达69.17%。固定化反胶团漆酶处理较游离漆酶处理的DDTs降解率提高了近20%。Laccase in reverse micelles was immobilized on modified diatomite by physical adsorption. The immobilization conditions and characterization of the immobilized reverse micelles laccase were investigated. The remediation of DDT-contaminated soil with immobilized reverse micelles laccase was studied. The optimal temperature was 35 ℃ and the optimal adding quantity of Tween-80 added to diatomite was 15% of the mass of diatomite for immobilization of laccase in reverse micelles. The activity of the immobilized reverse micelles laccase was maximal at 35℃ and pH 3.5-5.0. Degradation rates of total DDT （DDTs） was only 50.53% in soils treated with free laccase but up to 69.17% in soils treated with the immobilized reverse micelles laccase. Compared with the free laccase, the degradation rates of DDTs using the immobilized reverse micelles laccase increased about 20%.

关 键 词：反胶团漆酶 固定化 稳定性 DDT 

分 类 号：Q93[生物学—微生物学]