RNAi靶向抑制HBV复制和HBeAg表达  

TARGETED INHIBITION OF RNAi ON HBV REPLICATION AND HBeAg EXPRESSION

作　　者：李绍祥[1] 闫彩珍[2] 张成平[1] 胡大荣[1] 范公忍[1] 刘超英[1] 

机构地区：[1]北京军区总医院肝病科,北京100700 [2]河北医科大学基础医药学院药理学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2008年第3期333-336,共4页Journal of Hebei Medical University

基　　金：国家自然科学基金资助项目(30471541);北京军区十一五计划课题(06BJ020)

摘　　要：目的观察靶向HBVC基因区的RNA干扰对HBV复制的抑制作用。方法选择HBV基因组C区的Nt2021-2049作为靶序列,合成相应的正、反义寡核苷酸,退火后形成双链,克隆入shRNA表达质粒,将得到的质粒与HBV质粒共转染HepG2细胞,观察HepG2细胞中HBV的受抑制情况。结果病毒复制及HBeAg的表达明显受到抑制。结论所选靶区通过RNA干扰可有效抑制HBV复制。Objective To observe the inhibition of RNA interference（RNAi） targeting the HBV core gene on HBV replication. Methods Nt2021-2049 of HBV genome was selected as the target sequence, the respective sense and antisense oligonucleotide were synthesized , annealed and was cloned into the shRNA expressing plasmid, which was then cotransfected the HepG2 cell with the HBV plasmid. Afterwards, the inhibition on HBV in the HepG2 cell was observed. Results Replication of HBV and expression of HBeAg were found inhibited significantly Conclusion The chosen target region is effective in inhibiting HBV from replication by RNAi.

关 键 词：肝炎病毒 乙型 肝炎E抗原 乙型 基因表达调控 RNA干扰 

分 类 号：R512.62[医药卫生—内科学]