溶胶-凝胶固定纳米光纤探针上生物活性分子的研究  

Immobilization of biomolecules on nano-tips of optic-fibers by sol-gels

作　　者：郑长柏[1] 张工力[1] 

机构地区：[1]暨南大学光电工程研究所,广东广州510632

出　　处：《光学仪器》2008年第2期70-74,共5页Optical Instruments

基　　金：教育部科技计划资助项目(105174);广东省自然科学基金资助项目(04010484)

摘　　要：为提高纳米光纤生物传感器的性能,研究了以溶胶-凝胶法将生物活性分子固定于纳米光纤探针上。首先以正硅酸乙酯(TEOS)为前驱体,制备了以葡萄糖氧化酶(GOD)为生物活性分子的溶胶,然后利用提拉法将GOD凝胶固定于纳米光纤探针上;讨论了固定化GOD活性及凝胶膜在纳米光纤探针上成膜的条件;最后通过扫描电子显微镜检测到薄膜均匀连续,生物探针尺寸小于1μm,并利用电子探针验证了GOD被固定于纳米光纤探针上。In order to improve the performances of the nano-optic-fiber bio-sensors, the immobilizations of biomolecules on the nano-tips of optic-fibers by sol-gels were studied. The sols doped with glucose oxidase （GOD） as the biomolecules were firstly fabricated using tetraethoxy silane （TEOS） precursor, and then the GOD gels were immobilized on the nanotips of the optic-fibers by the lifting method. The conditions of retaining bioactivity of immobilized GODs and forming of gel films on the nano-optic-fibers were discussed. The results showed that the immobilized films were uniformity and the diameters of the tips were less than 1μm by the scanning electron microscope （SEM） and the GODs were examined to be immobilized on the nano-tips of optic-fibers by the electron-probe microanalyzer （EPMA）.

关 键 词：溶胶-凝胶 纳米光纤探针 膜的质量 生物活性 

分 类 号：TN25[电子电信—物理电子学] TP212.3[自动化与计算机技术—检测技术与自动化装置]