改造盘磨机对提高喜树碱提取得率的研究  

Research on Improving the Extraction Yield for Camptothecin by Reconstruction of Refiner

作　　者：孙玉[1,2] 房桂干[1,2] 王成章[1,2] 施英乔[1,2] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]国家林业局林产化学工程重点开放性实验室,江苏南京210042

出　　处：《生物质化学工程》2008年第3期17-20,共4页Biomass Chemical Engineering

基　　金：国家“十一五”科技支撑计划(2006BAD18B0503)

摘　　要：采用盘磨设备从喜树果实中提取喜树碱和10－羟基喜树碱，并对盘磨机进行了技术改造，增设了不锈钢料仓和中浓泵，实现了循环进料，主轴转速由1850r／min提高到5000r／min。比较了盘磨机改造前后从喜树果实中提取喜树碱和10-羟基喜树碱的效果，并通过正交试验进一步考察了喜树碱得率的影响因素。结果表明：盘磨设备经过改造后，喜树碱的得率相对提高14．81％～35．29％、10－羟基喜树碱的得率相对提高24．00％～44．44％，喜树碱得率达到0、06％，10-羟基喜树碱的得率达到0．04％。正交试验结果表明，NaOH溶液质量分数影响最为显著。In this research refiner is used to extract camptothecin（CPT） and 10-hydroxycamptothecin（HCPT） from the fruits of Camptotheca acuminata. The refiner has been reconstructed, adding mid-level concentration feeding pump, the rotate speed of principal axis has been increased to 5 000 r/min from 1 850 r/rain. The contents of camptothecin and 10-hydroxycamptothecin are determined by HPLC. It can be seen that the extraction ratio of camptothecin has been increased by 14.81%-35.29 % and that of 10-hydroxycamptothecin has been increased by 24.00 %-44.44 %. The yield of CPT is 0.06 % and the yield of HCPT is 0.04 %. The results of orthogonal test show that the mass fraction of sodium hydroxide solution has a significant effect.

关 键 词：善树碱 10-羟基喜树碱 盘磨机改造 高效液相色谱法 

分 类 号：TQ464.4[化学工程—制药化工]