发育期锌缺乏对小鼠脑金属硫蛋白表达的影响  

EFFECT OF DEVELOPMENTAL ZINC DEFICIENCY ON BRAIN METALLOTHIONEIN EXPRESSION IN ADULTS MICE

作　　者：郭俊生[1] 

机构地区：[1]第二军医大学军队卫生学教研室

出　　处：《上海铁道大学学报（医学版）》1997年第4期267-269,共3页

基　　金：国家自然科学基金;博士研究生基金

摘　　要：目的观察发育期锌缺乏小鼠其成年时脑金属硫蛋白（ｍｅｔａｌｏｔｈｉｏｎｅｉｎ，ＭＴ）的表达情况，探讨发育期锌缺乏影响脑功能及发育的机制。方法成年怀孕ＩＣＲ小鼠随机分为５组，各组动物在怀孕开始至出生后第２０天（Ｐ２０）进食不同锌水平实验饲料，使５组分别成为：严重缺锌组（ｓｅｖｅｒｅｚｉｎｃｄｅｆｉｃｉｅｎｃｙ，ＳＺＤ，饲料含锌量１ｍｇ／ｋｇ）、边缘缺锌组（ｍａｒｇｉｎａｌｚｉｎｃｄｉｆｉｃｉｅｎｃｙ，ＭＺＤ，饲料含锌量５ｍｇ／ｋｇ）、适锌组（ａｄｅｑｕａｔｅｚｉｎｃｓｕｐｐｌｙ，ＡＺＳ，饲料含锌量３０ｍｇ／ｋｇ），高锌配对组（ｈｉｇｈｚｉｎｃｐａｉｒ－ｆｅｄ，ＨＺＰ，饲料含锌量１００ｍｇ／ｋｇ）及高锌组（ｈｉｇｈｚｉｎｃｓｕｐｐｌｅｍｅｎｔａｔｉｏｎ，ＨＺＳ，饲料含锌量１００ｍｇ／ｋｇ）。然后各组实验动物均进食普通市售饲料至成年（７０日龄，Ｐ７０）。采用蛋白印迹杂交技术检测各组动物（Ｐ７０）脑组织ＭＴ的表达量。结果各组实验动物成年（Ｐ７０）脑组织均有ＭＴ印迹，脑ＭＴ表达量的顺序为ＳＺＤ＜ＭＺＤ＜ＡＺＳ＜ＨＺＰ＜ＨＺＳ；ＳＺＤ组动物脑ＭＴ的表达量仅为ＨＺＳ组的１９．４％，ＭＺＤ组动物脑ＭＴ的表达量亦只有ＨＺＳ组的?Objective To examine the brain metallothionein expression of developmental zinc deficiency mice in adult,and to study the mechanisms of decelopmental zinc deficiency on the function and deveopment of brain. Methods Of the first day of pregnancy,maternal ICR strain mice were randomly allocated into 5 groups:SZD (Severe Xinc Deficiency,1mg Zn/kg diet),MZD (Marginal Zinc Deficiency,5mg Zn/kg diet),AZS (Adequate Zinc Supply,30Zn/kg diet),HZP (High Zinc PairFed,100mg/kg diet) and HZS (High Zinc Supplementation,100mg/kg diet).From embryonic day (EO) to postnatal day 20 (P20),maternal mice were fed with experimental diets,then up to adult (70 of day age,P70),all pups in experimental groups were fed with normal diet.The brain metallothionein of experimental mice were examined with Western blotting on P70. Results There were metallothionein immunostainings in brain of all experimental groups.The concentration of brain metallothionein showed:SZD group<MZD group<AZS group<HZP group<HZS group. Conclusion Reduction of adult brain metallothionien expression might result from developmental zinc deficiency and that might be one of the mechanisms of brain function damage.

关 键 词：锌缺乏病 脑 金属蛋白 蛋白印迹 

分 类 号：R591.1[医药卫生—内科学] R151.1[医药卫生—临床医学]