玉米和类玉米中45S rDNA位点的CPD显带识别  

Identification of 45S rDNA sites in maize and teosintes by CPD Banding

作　　者：韩永华[1] 刘金花[1] 金危危 

机构地区：[1]中国农业大学国家玉米改良中心/农业部作物基因组学与遗传改良重点实验室

出　　处：《中国农业大学学报》2008年第3期19-22,共4页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30771208)

摘　　要：采用CPD(PI和DAPI组合)染色对玉米和类玉米有丝分裂中期染色体进行了显带分析,供试物种所有的45S rDNA位点都能够有效地被CPD染色显示为红色带纹。在栽培玉米、墨西哥玉米、二倍体多年生类玉米中只有一对染色体上具有45S rDNA位点,在多年生类玉米的2对染色体上具有45S rDNA位点。In this study, we performed combined PI and DAPI stain teosintes. Our results revealed that all of the 45S rDNA sites in the by CPD staining. 45S rDNA was only detected on one chromosome rennis, while in Zea perennis, 45S rDNA was detected on two pa ng on mitotic metaphase chromosomes in maize and species tested were efficiently shown as red bands pair in Zea mays ssp. mexicana and Zea diplopers of chromosomes.

关 键 词：玉米 类玉米 CPD(PI和DAPI组合)染色 核仁组织区(NOR) 

分 类 号：Q343.2[生物学—遗传学]