六神丸精简方抗炎镇痛活性的初步研究  

Preliminary Studies on Anti-Inflammatory and Analgesic Activities of Optimized Liushen Wan(CNZ)

作　　者：王清正[1] 寇俊萍[1] 马宏跃[1] 沈花[1] 严永清[1] 余伯阳[1] 

机构地区：[1]中国药科大学中药复方研究室,南京210038

出　　处：《中药新药与临床药理》2008年第3期180-183,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省教育厅高技术项目(JH03-014)。

摘　　要：目的研究六神九精简方(CNZ)的抗炎、镇痛活性,为将其研制成抗上呼吸道炎症的新药提供一定的实验依据。方法采用醋酸致小鼠腹腔毛细血管通透性升高模型和角叉菜胶致小鼠足跖肿胀模型,考察其抗炎作用;采用醋酸致小鼠扭体模型和热刺激致疼痛模型,考察其镇痛活性,并和六神丸原方进行比较。结果六神丸精简方CNZ 16,32,64 mg/kg三个剂量组单次灌胃给药,均可显著地抑制醋酸致小鼠腹腔毛细血管通透性的升高;其中,CNZ中、高剂量组可明显抑制角叉菜胶引起的小鼠足跖肿胀,注射后3 h达到作用高峰,并可持续到5 h以上。三个剂量组均显著减少醋酸诱导的小鼠扭体次数;中、高剂量组单次给药后0.5 h,即可显著延长热刺激引起的小鼠痛阈,作用可持续3 h以上,与六神丸原方作用相当。结论六神丸精简方CNZ具有显著的抗炎、镇痛活性。Objective To observe the anti- inflammatory and analgesic effects of CNZ, an optimized prescrition of Liushen Wan based on our previous studies, so as to provide some pharmacological evidence for its further clinical use. Methods Both acetic - acid - induced increased mouse vascular permeability and carrageenan - induced mouse footpad edema were used to study the anti - inflammatory activity of CNZ. Meanwhile, its analgesic activity was evaluated in mice model of pain induced by thermal stimulus and acetic acid. The above activities were compared with those of Liushen Wan. Results CNZ significantly reduced acetic - acid - induced dye leakage at the doses of 16, 32 and 64mg/kg aftersingle oral administration. Meanwhile, CNZ at the two higher doses also markedly inhibited carrageenan - induced footpad edema, which exerted the strongest effect at 3 hours after carrageenan injection, and lasted over 5 hours. On the other hand, CNZ remarkably suppressed acetic - acid - induced writhing response at low, moderate - and high - doses, and significantly prolonged pain threshold in hot plate assay at moderate - and high - doses 0.5 hour after oral adminis- tration, lasting over 3 hours. Overall, its potency was similar to that of Liushen Wan. Conclusion CNZ has significant anti - inflammatory and analgesic activities.

关 键 词：六神丸精简方(CNZ) 抗炎 镇痛 

分 类 号：R285.5[医药卫生—中药学]