活血抗生滴丸治疗佐剂型关节炎大鼠的机制研究  

Mechanism Study of Huoxue Kangsheng Dripping Pills in Treating Adjuvant-induced Arthritis Rats

作　　者：王淑美[1] 徐晓玉[2] 张文亮[3] 陈刚[4] 胡益勇[3] 

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]西南大学药学与中医药学院,重庆400715 [3]重庆医科大学,重庆400016 [4]重庆工商大学药物化学与化学生物学研究中心,重庆400067

出　　处：《中药新药与临床药理》2008年第3期197-200,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：重庆医科大学创新基金(重医大科[2005]8号)。

摘　　要：目的观察活血抗生滴丸对佐剂型关节炎(AA)大鼠血清、滑膜血管内皮生长因子/内皮抑素(VEGF/en- dostatin)失衡的影响。方法建立大鼠AA模型,将Wistar大鼠随机分为活血抗生滴丸低、中、高(0.63,1.25,2.5 g·kg^(-1))剂量组、环孢霉素A组(10 mg·kg^(-1))及模型组。于造模后第19天开始给药,每日1次,连续灌胃30 d。检测各组大鼠关节肿胀度、关节炎指数积分。ELISA法检测各组大鼠血清VEGF、endostatin蛋白的表达。免疫组化法检测各组大鼠滑膜组织VEGF、endostatin蛋白的表达。计算VEGF/endostatin比值。结果活血抗生滴丸高剂量组和环孢霉素A组可降低大鼠关节肿胀度、关节炎指数积分,降低大鼠血清和滑膜VEGF含量,提高endostatin含量,降低VEGF/endostatin比值,与模型组比较,差异有显著性(P<0.05或P<0.01)。结论活血抗生滴丸可抑制AA大鼠炎症发展,其机制可能与活血抗生滴丸调控VEGF/endostatin的失衡而抑制滑膜组织血管生成相关。Objective To investigate the VEGF/endostatin in serum and synovium effects of Huoxue Kangsheng （HXKS） Dripping Pills on the imbalance of of adjuvant -induced arthritis （AA） rats. Methods Wistar rats with adjuvant - induced arthritis were treated with HXKS Dripping Pills O. 63, 1.25, 2.5 g · kg^-1 and cyclosporine A 10 mg · kg^-1 respectively on the 19th day, ig, qd, for 30 days. The therapeutic effects were evaluated by the measurement of joints swelling and arthritis index. The expression of VEGF and endostatin in serum and synovium was assayed by ELISA and immunohistochemical tests respectively. Results Compared with AA models, HXKS Dripping Pills 2.5 g · kg^-1 group and cyclosporine A group remarkably alleviated joints swelling and reduced the arthritis index. HXKS Dripping Pills 2. 5 g · kg^-1 and cyclosporine A also reduced the expression of VEGF, increased the expression of endostatin and reduced the ratio of VEGF/endostatin in serum and synovium （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion HXKS Dripping Pills can attenuate the degree of chronic inflammation and angiogenesis in synovium of AA rats. The mechanism might be associated with the adjustment to the imbalance of VEGF/endostatin in synovium of AA rats.

关 键 词：活血抗生滴丸 佐剂型关节炎 血管内皮生长因子 内皮抑素 

分 类 号：R285.5[医药卫生—中药学]