抗-HIV不同检测方法的结果比较  

Result comparison of different anti-HIV antibody detection methods

作　　者：胡京辉[1] 王瑞[1] 孙莉[1] 

机构地区：[1]北京市血液中心检验科,100088

出　　处：《检验医学与临床》2008年第11期656-657,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨不同检测方法、不同检测试剂对献血者人类免疫缺陷病毒抗体(抗-HIV)检测结果的影响。方法采用不同试剂使用酶联免疫吸附试验(ELISA)对献血者血浆标本进行抗-HIV分析,呈反应性血浆标本采用免疫学蛋白印迹法进行确证分析。结果ELISA再次检测抗-HIV阳性率显著低于初次检测阳性率,差异有统计学意义(P<0.01),确证试验阳性率显著低于初次检测和再次检测的阳性率,差异有统计学意义(P<0.01)。结论采用不同试剂检测抗-HIV对于保证血液安全是非常必要的。Objective To investigate the impact of different detection methods and reagents on testing result of anti-HIV antibody in blood donors. Methods Anti-HIV antiboby was detected with ELISA using different reagents in blood donors, and positive sera were examined by Western blotting confirmatory test. Results The positive rate of anti-HIV by confirmatory test was significant lowed than that by ELISA（P〈0.01）. Conclusion It is very important to adopt different reagents in testing anti-HIV in blood donors for blood safety.

关 键 词：艾滋病 人类免疫缺陷病毒抗体 酶联免疫吸附试验 

分 类 号：R512.91[医药卫生—内科学]