子宫内膜异位症患者腹腔液一氧化氮对人精子活力的影响  

Impact of NO in Peritoneal Fluid on Sperm Motility in Patients with Endometriosis

作　　者：罗利[1] 谭世桥[1] 徐雁飞[1] 

机构地区：[1]四川大学华西第二医院生殖内分泌科,成都610041

出　　处：《四川大学学报（医学版）》2008年第3期427-429,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的了解子宫内膜异位症(EMS)患者腹腔液一氧化氮(NO)对人精子活力的影响,探讨NO与EMS妇女低妊娠率之间的关系。方法通过腹腔镜抽取EMS组、EMS合并不孕组及对照组腹腔液,分离、培养腹腔液中的巨噬细胞,检测细胞培养液中NO代谢物(硝酸盐和亚硝酸盐)浓度,将细胞培养液与精液共同孵育后检测精子的活力。结果EMS组和EMS合并不孕组细胞培养液NO代谢物水平高于对照组,差异有统计学意义(P<0.05);EMS合并不孕组患者细胞培养液与精液孵育后精子运动指标低于对照组与EMS组(P<0.05),对照组与EMS组之间精子运动指标差异无统计学意义(P>0.05)。结论EMS合并不孕患者腹腔液巨噬细胞培养液对精子活力有抑制作用,但未能证实与腹腔液中NO水平有关。Objective To determine the level of NO in the peritoneal fluids in patients with endometriosis and to evaluate the impact of NO on human sperm motility. Methods Peritoneal fluids were taken from 19 patients with Endometriosis, 16 infertile patients with Endometriosis, and 21 patients without Endometriosis （control group）. The macrophages in the peritoneal fluids were segregated and cultivated, The concentrations of NO metabolin （nitrate and nitrite） were measured. Human sperms were incubated with the culture fluids to detect the motility of the sperms. Results The patients with Endometriosis had significant higher levels of NO in the peritoneal fluids than the controls （P^0. 05）. The motility of sperms declined significantly in the culture fluids of infertile patients with Endometriosis compared with the fertile patients with Endometriosis and the controls （P〈 0. 05）. Conclusion Elevated NO in the peritoneal fluids in patients with endometriosis may play a role in the pathogeneses of Endometriosis-associated infertility.

关 键 词：子宫内膜异位症 腹腔液 一氧化氮 精子活力 

分 类 号：R711.71[医药卫生—妇产科学]