窄谱中波紫外线和/或阿维A对角质形成细胞异维A酸受体α mRNA表达的影响  

Alteration of Retinoid X Receptor α mRNA Level after Acitretin and/or Narrow-band UVB Treatment in Normal Human Keratinocytes

作　　者：罗素菊[1] 郑焱[1] 彭振辉[1] 王国荣[2] 周少娜[1] 李晓莉[1] 

机构地区：[1]西安交通大学第二医院皮肤科,西安710004 [2]陕西省人民医院普通外科

出　　处：《四川大学学报（医学版）》2008年第3期455-457,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30371295,30400387);陕西省自然科学基金(批准号2004C211)资助

摘　　要：目的研究阿维A和/或窄谱中波紫外线（NB-UVB）对培养的正常人角质形成细胞异维A酸受体α（RXRα）mRNA表达的影响。方法用10^-7 - 10^-6mol/L阿维A和/或50-100mJ/cm^2NB-UVB处理正常人角质形成细胞后,继续培养12h,用RT-PCR和实时荧光定量RT-PCR法检测角质形成细胞RXRα mRNA表达的改变。结果50-100mJ/cm^2NB-UVB单独照射角质形成细胞后,可抑制RXRα mRNA的表达;10^-7 -10^-6mol/L阿维A单独处理后,RXRα mRNA的表达无明显降低,二者联合作用时,对RXRα mRNA的表达抑制作用更强。结论NB-UVB单独作用可明显抑制RXRα mRNA的表达,阿维A单独处理对RXRα mRNA的表达无明显影响,但二者联合作用时对RXRα mRNA的表达存在协同抑制作用。Objective To investigate the alteration of retinoid X receptor α（RXRα） mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment. Methods After a 12-hour incubation with 10^-7 - 10^-6 mol/L acitretin and/or following 50-100 mJ/cm^2 NB-UVB irradiation in normal human keratinocytes, RXRa mRNA expression was examined by reverse transcription polymerase chain reaction （RT- PCR） and real-time quantitative RT-PCR. Results The expression of RXRa mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRa mRNA expression was observed than that of single treatment. Conclusion Narrow-band UVB irradiation treatment can decrease RXRa mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.

关 键 词：角质形成细胞 维A酸受体 维A酸 中波紫外线 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]