河南省唐河县分离到基因1型乙型脑炎病毒  

Isolation and identification of Japanese encephalitis virus in Tanghe county, Henan Province

作　　者：王环宇[1] 郝宗宇[2] 付士红[1] 张爱梅[2] 曹玉玺[1] 宋付党 李林红[2] 何英[1] 王焕琴[1] 唐青[1] 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控锅所病毒性脑炎室 传染病预防控制国家重点实验室,北京100052 [2]河南省疾病预防控制中心 [3]河南省南阳市唐河县疾病预防控制中心

出　　处：《中华实验和临床病毒学杂志》2008年第2期83-86,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：国家科技部重大传染病专项（2003BA12A08.01）;本研究受中日传染病项目资助（Japanese Science and Health Foundation）

摘　　要：目的从河南省唐河县采集的蚊虫标本中分离乙型脑炎病毒（JEV）并确定其基因分型及E基因区段氨基酸序列特征。方法对2004年采集蚊虫标本进行病毒分离，对新分离的乙脑病毒进行生物学、血清学及分子生物学鉴定。逆转录聚合酶链反应（RT-PCR）扩增新分离JEV的PrM、E区段核苷酸序列，测序后应用Clustal X软件做碱基配对分析，MEGA3．1完成病毒进化分析，GENEDOS（3．2）软件完成氨基酸位点分析。结果共采集3722只蚊虫标本，包括：库蚊、骚扰阿蚊、伊蚊及按蚊。从库蚊标本中分离到3株属于基因1型的乙脑病毒，E区段核苷酸和氨基酸与减毒活疫苗株SA14-14-2株的同源性分别为86．9％-87．7％，氨基酸同源性为95．2％-97．0％，存在12处共同的氨基酸位点差异。结论从河南省唐河县首次分离到基因1型的乙脑病毒。E基因与疫苗株相比有部分氨基酸差异，但现行疫苗株理论上可以保护新分离乙脑病毒。Objective To isolate Japanese encephalitis virus （JEV） from mosquitoes collected in Tanghe county, Henan province and analyze the genotype of the newly isolated JEV strains and the characteristics of amino acid in the E gene. Methods Viruses were isolated from mosquitoes collected in 2004 and identified by biological, serological and molecular biological methods. PrM and E segments of the newly isolated JEV were amplified by RT-PCR, the PCR products were purified and sequenced. Multiple alignment, phylogenetic and amino acid （AA） analysis were carried out by Clustal X （ 1.8 ） program, MEGA 3.1 and GENEDOS （ 3.2 ）. Resulls Totally 3722 mosquitoes were collected including Culex, Armigeres, Aedes, Anopheline. Three new JEV strains isolated from Culex belonged to genotype 1. The homologue of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 was 86.9%-87.7% and 95.2%-97.0%, respectively. Totally there were 12 common sites of amino acid differences in E gene between them. Conclusion Newly isolated viruses in Henan province belonged to JEV genotype 1. It suggests that the vaccine strain SA14-14-2 currently used for preventing JE is able to protect people from JEV infection, although there are some amino acid differences between them.

关 键 词：脑炎病毒 日本 基因分型 种系发生 

分 类 号：R373[医药卫生—病原生物学]