人博卡病毒感染支气管上皮细胞生物学特征的研究  

Characterization of the cytopathic efect in human bronchial epithelial cell after human bocavirus infection（HBoV）

作　　者：林峰[1] 侯建毅[1] 郑敏巧[1] 吴锋[1] 曾爱平[1] 李桦[1] 郑昌华[1] 陈弘[1] 李旭阳[1] 饶高峰[1] 莫一涵[1] 黄恩佩[1] 

机构地区：[1]温州医学院附属温岭医院,浙江温州317500

出　　处：《中华实验和临床病毒学杂志》2008年第2期107-109,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：国家自然科学基金（No.30771913）;国家高技术研究发展“八六三”计划基金（No.2007AA02Z400）

摘　　要：目的研究人博卡病毒（HBoV）在呼吸道感染中的致病性及机制。方法采用人博卡病毒阳性标本感染健康人支气管上皮细胞，观察HBoV感染支气管上皮细胞后的生物学特征，并进行病毒空蚀斑、红细胞吸附抑制及免疫荧光测定。结果75％感染细胞变圆并堆积成葡萄状，伴随有坏死、破碎、脱落。病毒蚀斑测定出典型“猫头鹰眼”现象。红细胞吸附实验观察到90％以上红细胞附着感染支气管上皮细胞。荧光检测出在细胞核内具有典型荧光块。结论支气管上皮细胞可用于分离HBoV，HBoV感染支气管上皮细胞后能够在该细胞内增殖，并导致特征性生物学改变。本研究为今后人博卡病毒致病机制研究奠定基础。Objective In this study, human bronchial epithelial cell were inoculated with positive sputum specimens of HBoV. Methods After four days infection, cytopathic effect （CPE） were observed by inverted microscopy. These viruses all cause typical cell damages such as rounded and shrivelled, fusion and fallout. Results These damages got quick following increased future degenerations. The other assay result of CPE within the infected cells were observed by inverted microscopy, have typical “owl＇s eye” plaque and above 90 percent hemadsorption within the infected cells by erthrocytes for hemadsorption technique. The typical fluorescence lump of nucleus within the infected cells was found by indirect immunofluorescence technique. Conclusion Isolation and identification of HBoV could be done in the human bronchial epithelial cell, and we found some characterizing CPE in the human bronchial epithelial cell after HBoV infection. The above studies pave a way for studying pathogenicty of human bocavirus.

关 键 词：细小病毒科 感染 上皮细胞 支气管 致细胞病变 病毒 

分 类 号：R373[医药卫生—病原生物学]