一氧化氮对球虫(E.tenella)卵囊孢子生殖的抑制特性  

The inhibitory effects of NO on the sporulation process of E.tenella oocysts

作　　者：李金贵[1] 江燕[1] 陶建平[1] 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医杂志》2008年第5期5-8,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金资助项目(30300259)

摘　　要：本研究利用NO供体以及几种抗氧化剂和NO清除剂来分析外源性NO对E.tenella卵囊孢子生殖的抑制特性。试验结果表明:S-亚硝基硫醇类NO供体(SNAP和GSNO)对卵囊的抑制作用具有明显的剂量-效应关系,8mmol/L的SNAP和GSNO均可完全抑制新鲜卵囊的孢子生殖;KCN对卵囊的抑制呈现为剂量-效应关系,但NaN3没有抑制作用。维生素C、甘露醇和水杨酸钠等抗氧化物质以及FeSO4和DTT均不能消除GSNO对卵囊孢子生殖的抑制作用。K2Cr2O7和KMnO4均会明显抑制GSNO对卵囊的作用。NO清除剂血红蛋白(Hb)能够消除NO供体对卵囊的抑制作用,并呈现剂量-效应关系。The NO-donors, several kinds of antioxidants and NO-scavengers were used to investigate the inhibitory effects of exogenous NO on the sporulation process of E. tenella oocysts. The results showed that the inhibitory effects of NO-donors, S-Nitrosothiols on oocysts had an obvious dose-effect relationship. Eight mmol/L SNAP or GSNO could inhibit the sporulation of freshly isolated oocysts. The inhibitory effects of KCN on oocysts also showed a dose-effect relationship, but oocysts were not inhibited, by NAN3. The antioxidants, such as VC, mannitol and sodium salicylate, could not eliminate the inhibitory effects of GSNO on oocysts, neither does the FeSO4 nor DTT. However, the oxidants, K2Cr2O7 and KMnO4, could obviously inhibit the effect of GSNO on oocysts. The NO-scavengers, hemoglobin, could significantly eliminate the inhibitory effects of NO on oocysts in a dose-dependent manner.

关 键 词：一氧化氮 柔嫩艾美耳球虫卵囊 孢子生殖 抑制特性 

分 类 号：S855.9[农业科学—临床兽医学]