外周血单核细胞亚群流式图谱分析  

Flow cytometry analysis of unusual peripheral monocyte population

作　　者：张立新[1] 叶军[1] 林梅[1] 陆桃红[1] 徐祖龙[1] 朱学骏[1] 陈亚宝[1] 

机构地区：[1]江苏省泰州市人民医院中心实验室,225300

出　　处：《中华检验医学杂志》2008年第5期504-508,共5页Chinese Journal of Laboratory Medicine

摘　　要：目的用流式细胞术分析外周血白细胞在前向角散射光强度（FSC）／侧向角散射光强度（SSC）散点图谱中单核细胞右下侧出现的一团细胞，并分析它出现的影响因素。方法采用流式细胞仪分析细胞表面CD分子以鉴定细胞；通过实验研究温度、溶血素浓度、时间对它的影响，并测定血脂生化指标，通过统计学方法观察它们之间的关系。结果（1）在FSC／SSC散点图谱中单核细胞右下侧出现的一团细胞与正常单核细胞表型同为CD45＋CD13＋CD14＋,CD3-CD319-；（2）应用溶血素前后对比，单核细胞位置在FSC／SSC散点图左移；（3）单核细胞在37℃时抵抗溶血素能力比22℃时降低；溶血时间延长，单核细胞左移增加；（4）患者出现单核细胞分群比例[31．5％（40／126）]高于健康对照者[5．1％（5／98）]（X2=22．74，P〈0．01）；（5）单核细胞分群标本的血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、载脂蛋白B100比不分群的标本低（P〈0．05），而总胆红素、白蛋白、高密度脂蛋白胆固醇、载脂蛋白A、脂蛋白a（Lpa）比较差异无统计学意义。结论单核细胞在FSC／SSC散点图谱中可分为两群，其出现与单核细胞膜对溶血素的抵抗能力有关；它受溶血时间和温度影响。因此在检测单核细胞相关标志和功能时，要注意分群现象和细胞膜异常可能带来的影响。Objective To analyze a population of cells on the fight lower lateral of monocyte population in forward scatter/side scatter （FSC/SSC） （X-axis/Y-axis） scatterplot of peripheral blood leucocyte by flow cytometry （FCM） and its influencing factors. Methods The type of cells were identified based on cluster of differentiation antigen （CD） by FCM. The impact of temperature, hemolysin concentration, and incubation time was evaluated. Blood lipid tests were performed to observe the relation between them by statistical methods. Results （ 1 ） Phenotype of this population of cells on the right lower lateral of monocytes in FSC/SSC scatterplot is CD45＋CD13＋CD14 CD3- CD19-, which was the same as monocyte cells; （2） The monocytes in FSC/SSC scatterplot shifted to left side after using haemolysin; （3） The monocytes showed less resistance to antibemolysin in 37℃ than that in 22℃ ; There were more monocytes shifting to left side with the increase of haemolysis time; （4）The swarming ratio of monocytes in patients （31.5% ,40/126） was higher than it in normal controls （ 5. 1%, 5/98 ） （X2 = 22. 74, P 〈 0. 01 ） ; （5） The levels of total serum cholesterol （ TC ）, triglyeride （ TG ）, low density lipoprotein cholesterol （ LDL-C ）, apoprotein B100（ Apo B100）in patients with swarming monocytes were lower than that in the patients without swarming monocytes, （ P 〈 0. 05 ）. There was no statistical significance between the two groups with respect to levels of total bilirubin （ TBIL）, albumin （ Alb）, high density lipopretein cholesterol （ HDL-C）, apopretein A Ⅰ （APO A Ⅰ ）, lipopretein （Lpa）. Conclusions Peripheral blood monocytes can be divided in two groups in FSC/SSC scatterplot when analyzed with FCM. The presence of this population of cell was related to resistance to hemolysin. It can be influenced by haemolysis time and incubation temperature. Therefore, the effect of swarming monocytes and abnormal cell membrane should be taken int

关 键 词：流式细胞术 单核细胞 细胞膜 溶血素类 HLA—DR抗原 

分 类 号：R686[医药卫生—骨科学]