利用门多萨假单胞菌NK-01合成中长链聚羟基脂肪酸酯(PHAMCL)  

Synthesis of Medium-chain-length Polyhydroxyalkanoate (PHA_(MCL)) by Pseudomonas mendocina NK-01

作　　者：陶剑[1] 郑承纲[1] 张斌[1] 郭文斌[1] 王淑芳[2] 宋存江[1] 

机构地区：[1]南开大学生命科学学院分子微生物学与技术教育部重点实验室,天津300071 [2]南开大学生命科学学院生物活性材料教育部重点实验室,天津300071

出　　处：《微生物学通报》2008年第5期782-787,共6页Microbiology China

基　　金：国家“863计划”目标探索类课题(No.2007AA06Z323);天津市科技攻关项目(No.06YFGZSH06800)

摘　　要：从土壤中分离到1株能够合成中长链聚羟基脂肪酸酯(PHAMCL)的细菌,经分类鉴定命名为Pseudomonas mendocina NK-01。对于该菌株在氮源限制的情况下,利用葡萄糖合成的PHAMCL进行了结构分析。同时,获得了该菌株PHA合成酶基因phaC1,利用大肠杆菌表达载体pBluescript SK-构建了重组载体pBSphaC1,并在E.coli JM109中成功表达。以所构建的工程菌株进行了发酵合成。A strain which can synthesize medium-chain-length polyhydroxyalkanoate （PHAMCL） was isolated from soil It was named as Pseudomonas mendocina NK-01 through classificatiort and identification. The PHAMCL was synthesized by P. mendocina NK-01 from glucose in the condition of limited nitrogen source. The structure of it was characterized. In order to construct a recombined plasmid pBSphaC1, an expressing plasmid of E.coli, after phaC1 gene encoding PHA synthase was cloned from Pseudomonas mendocina NK-01 by PCR amplification, it was linked into pBluescript SK-. PhaC1 gene was expressed successfully in E.coli JM109. Fermentation synthesis was also investigated by recombined E.coli,

关 键 词：中长链聚羟基脂肪酸酯 门多萨假单胞菌 PHA合成酶 

分 类 号：Q939.9[生物学—微生物学]