致肾盂肾炎大肠杆菌毒力因子的分布及其与耐药性的关系  

Relationship between Virulence Factor of Pyelonephritic E. coli and Antibiotic Resistance

作　　者：侯敏[1] 贺靖冬[1] 陈锦英[2] 张连祥[1] 刘晨梅[1] 

机构地区：[1]天津市胸科医院,天津医科大学基础医学院300051 [2]天津医科大学基础医学院

出　　处：《天津医药》2008年第5期321-323,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30470096)

摘　　要：目的:探讨致肾盂肾炎大肠杆菌毒力因子的分布及其与耐药性的关系。方法:用PCR和多重PCR方法检测81株尿源大肠杆菌的毒力因子基因papC、fimH、hly、cnf1和aer等;用K-B法进行15种抗菌药物的敏感试验。结果:papC阳性41株(50.6%),为致肾盂肾炎大肠杆菌(UPEC)组,papC阴性40株(49.4%),为非UPEC组。UPEC组hly、aer、fimH、cnf1的检出率依次为63.4%、61.0%、53.7%和34.2%;非UPEC组依次为10.0%、35.0%、12.5%和5.0%;2组之间4种毒力因子的检出率差异均有统计学意义(χ2分别为24.746、7.977、10.281和9.085,P<0.05或P<0.01)。总耐药率为87.65%,UPEC组和非UPEC组的多重耐药率之间差异有统计学意义(P<0.01),前者(87.8%)明显高于后者(50%)。结论:UPEC菌株毒力因子检出率高,多重耐药性突出,应引起临床上的高度重视。Objective： To study the distribution of the virulence factor and its relationship with the antibiotic resistance of the pyelonephritic E.coli（UPEC）. Methods： The virulence factors,papC,fimH,hly,cnfl and aer gene of the 81 strains of E.coli isolated from urine were tested using PCR and multiple PCR. The sensitivity tests of 15 drugs were detected by K-B methods. Results： The positive rate of the papC was 50.6% in UPEC strains. The negative rate of the papC was 49.4% in non-UPEC strains. Among the UPEC strains, positive rates for hly,aer,fimH and cnfl were 63.4% ,61.0% ,53.7% and 34.15%, respectively. Among the non-UPEC strains those positive rates were 10.0%, 35.0%, 12.5% and 5.0% respectively （χ2 = 24.746,7.977,10.281 and 9.085, P 〈 0.05 or P 〈 0.01 ）. Drug-sensitivity result showed that the total resistant rate was 87.65%. There were statistical differences between the UPEC strains（ 87.8% ） and the non-UPEC strains（50% ） in multiple antibiotic resistant rates （P 〈 0.01 ）. Conclusion： It is important to pay attention to the higher positive rate of UPEC and serious multiple antibiotic resistance clinically.

关 键 词：肾盂肾炎 大肠杆菌 聚合酶链反应 毒力因子类 微生物敏感性试验 

分 类 号：R392-33[医药卫生—免疫学] R978.1[医药卫生—基础医学]