介入给予左旋精氨酸及硝酸甘油对大鼠急性局部脑缺血模型的作用  

Effects of interventional administration of L-Arginine or nitroglycerin on acute local cerebral ischemia in rats

作　　者：朴翔宇[1] 孙长凯[2] 马辉[2] 周佩洋[2] 

机构地区：[1]大连大学附属中山医院神经内科,辽宁大连116001 [2]大连医科大学脑疾病研究所,辽宁大连116044

出　　处：《基础医学与临床》2008年第5期423-427,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30570464)

摘　　要：目的通过介入手段局部给予一氧化氮(NO)前体L-精氨酸(L-ARG)、NO供体硝酸甘油(NG),观察对大鼠局灶性脑缺血的疗效。方法采用线栓法阻塞右侧大脑中动脉,制作缺血-再灌注大鼠模型(MCAO),42只雄性SD大鼠随机分为4组:MCAO模型组(n=12),SHAM假手术组(n=6),NG组(n=12),L-ARG组(n=12),于再灌注即刻患侧颈内动脉内分别给予NS、NS、NG以及ARG,再灌3、24 h各分为2个亚组,分别检测各组不同时间点Longa评分,血清NO氧化产物,以及脑组织苏木素-伊红(HE)染色,免疫组织化学染色。结果脑皮层和海马CA3区OX-42阳性细胞数:MCAO组再灌3 h OX-42染色加深,细胞形态基本清楚。24 h细胞明显深染,数量明显增加(P<0.001)。NG组3和24 h,细胞数量少(P<0.05)。L-ARG组3和24 h细胞数量亦减少(P<0.05)。结论NO前体L-ARG、供体NG介入给药对大鼠急性局灶性脑缺血具有保护作用。Objective To observe the effects of treating acute ischemic stroke in rats with interventional administration of nitric oxide precurcer L-Arginine or nitric oxide donor nitroglycerin. Methods The right middle cerebral arteries of rats were occluded by insertion of a suture to duplicate ischemia-reperfusion models. Forty-two male SD rats were randomly divided into four groups： MCAO group（ n = 12） ;sham operation group（n = 6） ; NG group （n = 12） and L-ARG group （n = 12） ,intracarotid arteries administrated respectively by NS,NS,NG and ARG. Each of the four groups were subdivided into 2 groups according to the reperfusion time （3 h and 24 h）, measurement of Longa scores, NO2 -/NO3 - in serum, HE staining and immunohistochemical （SABC）method were utilized to assess the changes of ischemic brain tissues in different groups. Results OX-42 positive cells of cortex and CA3 area of hippocampal： OX-42 positive cells were found, their features identified at 3 h after reperfusion. 24 h the response of microglias was obvious, the number of the cells increased （P 〈0. 001 ）. NG group 3 h and 24 h, the microglias could be found unclearly, the number was little （ P 〈 0.05）. L-ARG group 3 h and 24 h, the number was alsolittle （ P 〈 0. 0 5 ） . Conclusion Interventional administration of NO precursor/donator has showed significant protective function to acute ischemia stroke in rats.

关 键 词：L-精氨酸 硝酸甘油 缺血性卒中 OX-42 

分 类 号：R743.3[医药卫生—神经病学与精神病学]