SDF-1/CXCR4轴活化诱导人内皮祖细胞增殖、迁移及管型形成  

Activation of SDF-1/CXCR4 axis induces proliferation,migration,and tubulogenesis of human endothelial progenitor cells

作　　者：陈飞兰[1] 张华蓉[1] 徐承平[1] 卞修武[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《基础医学与临床》2008年第5期428-431,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30370552)

摘　　要：目的观察趋化因子SDF-1促内皮祖细胞增殖、迁移和管型形成的作用。方法用免疫细胞化学检测内皮祖细胞SDF-1和CXCR4表达;用MTT法、Millicell趋化法及Matrigel体外三维成型法分别检测不同浓度的趋化因子SDF-1促内皮祖细胞增殖、迁移和管型形成。并应用CXCR4受体抑制剂AMD3100观察上述指标的变化。结果免疫细胞化学显示内皮祖细胞表达SDF-1和CXCR4蛋白。SDF-1可促进内皮祖细胞的增殖、迁移和体外小管样结构的形成。AMD3100可抑制SDF-1的诱导作用。结论SDF-1/CXCR4轴在内皮祖细胞参与血管新生中可能发挥重要作用。Objective To explore the effect of chemokine SDF-1 on the proliferation, migration and in vitro of human endothelial progenitor cells （EPCs）. Methods The expression of CXCR4 and SDF-1 on EPCs was detected with immunocytochemistry. Proliferation, migration and in vitro tubulogenesis of EPCs was detected by MTI＇, Millicell chemotaxis and three-diamensional in vitro Matrigel assays, respectively. Results SDF-1 and CXCR4 were expressed on EPCs. SDF-1 induced proliferation, migration and tubulogenesis of EPCs. However, AMD3100 abolished the effects induced by SDF-1. Conclusion The SDF-1/CXCR4 axis possibly plays a critical role in regulating vasculogenesis of EPCs.

关 键 词：内皮祖细胞 趋化因子SDF-1 血管新生化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]