TRAF2在人B淋巴细胞AP-1信号传导系统中的作用  

Role of TRAF2 in AP-1 signaling pathway of human B cells

作　　者：张文[1] 张烜[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院风湿免疫科,北京100730

出　　处：《基础医学与临床》2008年第5期465-468,共4页Basic and Clinical Medicine

基　　金：人事部留学人员科技活动项目择优资助(M334600)

摘　　要：目的利用人B细胞株模型探讨TRAF2在调控AP-1信号系统中的作用。方法将融合有黄色荧光素(YFP)的野生型(WT-TRAF2)或功能缺失型(DN-TRAF2)TRAF2质粒,以及小干扰RNA-TRAF2质粒转染至人类B淋巴细胞株,过夜培养后经流式细胞仪或抗生素G418筛选阳性转染细胞,通过Western blot、ELISA等方法研究TRAF2对AP-1通路中ERK、JNK、P38磷酸化和AP-1亚单位的细胞核内转移等的影响。结果B细胞过度表达WT-TRAF2可增加ERK和P38的磷酸化水平以及C-FOS的核内转录,而过度表达DN-TRAF2或转染小干扰RNA-TRAF2则减少ERK和P38的磷酸化水平以及C-FOS的核内转录。结论TRAF2可选择性地作用于人B淋巴细胞AP-1信号传导系统中的部分激酶,对B细胞AP-1信号系统的活化有重要作用。Objective To investigate the role of TRAF2 in AP-1 signaling pathway of human B cells. Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion wild type TRAF2 （YFP-WT-TRAF2） or YFP fusion dominant-negative TRAF2 （ DN-TRAF2）, or transfected with shRNA-TRAF2 and control shRNA plasmid. After incubation overnight, cells were either sorted with flowcytometry or screened by antibiotics G418. Activation of AP-1 pathway, including phosphorylation of ERK, JNK and P38, as well as nuclear translocation of AP-1 subunits were detected by western blot and ELISA. Results Overexpression of WT-TRAF2 selectively induced activition of MAPK by phosphorylation of ERK and P38, and further induced nuclear translocation of C-FOS. Moreover, both overexpression of DN-TRAF2 and transfection of shRNA-TRAF2 inhibited phosphorylation of ERK and P38, and nuclear translocation of C-FOS. Conclusion TRAF2 selectively activates some kinases in CD40 mediated AP-1 signaling pathway, and plays an important role in AP-1 activation.

关 键 词：人B淋巴细胞 肿瘤坏死因子受体相关因子2 AP-1信号通路 

分 类 号：R392[医药卫生—免疫学]