甘草酸二铵对慢性病毒性心肌炎小鼠心肌中性粒细胞浸润及细胞间黏附分子-1表达的影响  

Effects of diammonium glycyrrhizinate on myocardial infiltration of polymorphonuclear neutrophils and expression of intercellular adhesion molecule-1 in chronic stage of viral myocarditis

作　　者：马路[1] 王忠卯[2] 李文强[2] 姜树军[1] 栾文霞[2] 李泱[3] 薛桥[1] 高伟[3] 

机构地区：[1]中国人民解放军海军总医院干二科东五病区,北京100037 [2]中国人民解放军第15医院内一科,乌苏833000 [3]中国人民解放军总医院心血管病研究所,北京100853

出　　处：《中国新药杂志》2008年第9期739-742,共4页Chinese Journal of New Drugs

摘　　要：目的:探讨细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)及中性粒细胞(poly- morphonuclear neutrophils,PMNs)浸润在慢性病毒性心肌炎过程中的作用及甘草酸二铵(甘利欣,diammonni glycyrrhizinatis,DG)对其的影响。方法:建立慢性病毒性心肌炎小鼠模型,取心肌用逆转录-聚合酶链反应(RT-PCR)法和免疫组织化学方法检测ICAM-1 mRNA及其蛋白表达水平,用酶法测定PMNs数,酶联免疫方法测定血浆白细胞介素-6(IL-6)的含量。结果:慢性病毒性心肌炎ICAM-1表达水平升高,PMNs增加,血浆IL-6水平升高;甘草酸二铵可抑制慢性病毒性心肌炎心肌ICAM-1的表达、PMNs的增加及血浆IL-6水平的升高。结论:ICAM-1参与、影响慢性病毒性心肌炎的炎症过程,甘草酸二铵可能通过抑制ICAM-1的表达而产生心肌保护作用。Objective： To investigate the effects of diammonium glycyrrhizinate （ganlixin） on the expression of intercellular adhesion molecule-1 （ICAM-1） and myocardial infiltration of polymorphonuclear neutrophils （PMNs） in chronic stage of viral myocarditis （VMC）. Methods： After VMC was established in mice, the expression of ICAM-1 mRNA and protein was detected by semi-quantitative RT-PCR and immunocytochemistry. The numbers of PMNs were determined by measuring MPO enzyme activity. The interleukin-6 level in plasma was detected by ElISA method. The results from mice with and without ganlixin were compared. Results： The expression of myocardial ICAM-1 mRNA, the levels of plasma ICAM-1 protein and interleukin-6, and the number of PMNs were increased in VMC mice. Ganlixin significantly attenuated the increased responses in VMC mice （P 〈 0.05 or P 〈 0.01 ）. Conclusion： ICAM-1 inducing adhesion and infiltration of PMNs is implicated in the development of chronic stage of VMC. Ganlixin may exert a protective effect on chronic VMC via blocking ICAM-1 expression.

关 键 词：甘草酸二铵 慢性病毒性心肌炎 中性粒细胞(PMNs) 细胞间黏附分子-1(ICAM-1) 白细胞 介素-6 

分 类 号：R285.5[医药卫生—中药学] R542.21[医药卫生—中医学]