铬诱导植物根细胞壁化学成分变化的FTIR表征  

FTIR Spectroscopic Characterization of Chromium-Induced Changes in Root Cell Wall of Plants

作　　者：张晓斌[1] 刘鹏[1] 李丹婷[1] 徐根娣[1] 蒋敏姣[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《光谱学与光谱分析》2008年第5期1067-1070,共4页Spectroscopy and Spectral Analysis

基　　金：浙江省科技攻关重点项目(2005C24011);环境修复生态健康教育部重点实验室开放基金项目(050204)资助

摘　　要：随着制革、电镀等行业的发展,铬污染已成为严重的环境问题而受到人们的关注。铬在植物体内主要积累于根部,植物根细胞壁化学变化与金属元素在植物体内的蓄积行为有密切关系。与其他方法比较,应用OMNI采样器-FTIR直接测定法表征细胞化学成分变化具有简便、快速和准确的优点。文章用FTIR对凤眼莲(Eichhornia crassipes)和空心莲子草(Alternanthera philoxeroides)根细胞壁进行了测定、分析和指认,并利用半定量分析方法研究了铬处理和对照处理下根细胞壁红外光谱的差异。结果表明,铬处理的凤眼莲和空心莲子草根细胞壁上的—OH向低频分别发生了20和6 cm-1的位移,同时凤眼莲的根细胞壁上—OH和自由羧基COO-等吸附位点数目较对照均有所增加,而空心莲子草则相反。说明这些基团与铬的结合密切相关,进一步解释了凤眼莲耐铬及其在根部积累铬的机理,说明FTIR在监测逆境诱导植物化学成分变化和环境绿色修复方面具有广泛的应用前景。Due to its wide industrial use, chromium is considered a serious environmental pollutant. Contamination of soil and water by chromium （Cr） is of recent concern. Chromium mainly accumulates in root in plants, and the change in compounds of the root cell wall have a close relation with the Cr accumulation. Compared with the other identification methods, the identification of the Chinese traditional and herbal drugs using Fourier transform infrared spectrometer with OMNI collector is simple and convenient, fast and accurate. In the present paper, the spectra of cell wall of Cr-treated root and control of Eichhornia crassipes and Ahernanthera philoxeroides were determined. Absorption peaks were identified to the corresponding functional groups and half-quantitative analysis was also used. The results showed that a significant shift of -OH absorption peaks can be seen when comparing the FTIR spectra of control and Cr-treated plants, and the absorbency of -OH and COO- groups went up in E. crassipes root cell wall while droped in A. philoxeroides root cell wall. It is suggested that -OH and COO- groups were referred in binding Cr^6＋ in aqueous solutions, and this may be included in the mechanism of Cr accumulation in E. crassipes roots. Therefore, FTIR spectrometry could be widely used to monitor changes in chemical composition of plant parts under stresses and environmental restoration.

关 键 词：傅立叶变换红外光谱 铬 细胞壁 

分 类 号：O657.3[理学—分析化学]