异型双功能交联剂SPDP对C-藻蓝蛋白的光谱影响  

Spectral Changes of C-Phycocyanin with Different Molar Ratios of SPDP

作　　者：颜世敢[1] 陈秀兰[1] 张熙颖[1] 周百成[1] 张玉忠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室

出　　处：《光谱学与光谱分析》2008年第5期1115-1117,共3页Spectroscopy and Spectral Analysis

基　　金：国家高技术研究与发展规划资助项目(2008AA09Z404);国家自然科学基金项目(40676078);山东省科技发展计划项目(2006GG2205009)资助

摘　　要：一步阴离子交换层析法由钝顶螺旋藻中高效制备高纯度的C-藻蓝蛋白,纯化的C-藻蓝蛋白最大吸收峰位于620 nm,室温最大荧光发射峰位于640 nm。用异型双功能交联剂SPDP对C-藻蓝蛋白进行蛋白质交联,不同摩尔比的SPDP对C-藻蓝蛋白溶液的吸收光谱和室温荧光发射光谱有显著影响。随着SPDP/C-藻蓝蛋白摩尔比的增加,C-藻蓝蛋白的吸光度和相对荧光强度均不同程度降低,且室温荧光发射峰由640nm蓝移至630 nm。光谱研究结果表明用SPDP对C-藻蓝蛋白进行蛋白质交联时SPDP/C-藻蓝蛋白的摩尔比应小于100,否则荧光强度和荧光特性将发生显著改变。Pure C-phycocyanin was prepared from Spirulina platensis using one-step anion-exchange chromatography. The C-PC obtained was with an absorption maximum at 620 tam and a fluorescence emission maximum at 640 nrn when excited by 580 nm. SPDP is an excellent heterobifunctional crosslinker for thiolating amines. Different molar ratios of SPDP have remarkable influence on the absorption and fluorescence spectra of C-phycocyanin. The absorption maximum and fluorescence emission maximum both decreased and blue-shifted from 640 nm to 630 nm as the molar ratios of SPDP increased. It was found that the molar ratios of SPDP to C-phycocyanin was not more than 100 was appropriate to tion and fluorescence spectra of C-phycocyanin. being conjugated with other biomoleeules from the absorp-tion and fluorescence spectra of C-phycocyanin.

关 键 词：C-藻蓝蛋白 SPDP光谱 

分 类 号：Q586[生物学—生物化学]