细胞分裂素对蝴蝶兰胚性愈伤组织诱导的影响  

Effect of Cytokinin on Embryogenic Callus Induction in Phalaenopsis

作　　者：刘福平[1] 陈移亮[1] 

机构地区：[1]福建省亚热带植物研究所,福建厦门361006

出　　处：《热带作物学报》2008年第1期42-46,共5页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金项目(C0410040)资助

摘　　要：分别以6-Benzylaminopurine(6-BA)和2,4-Dichloro phenoxyacetic acid(2,4-D)诱导蝴蝶兰外植体。6-BA从嫩茎切块诱导出胚性愈伤组织,细胞富含淀粉粒,而2,4-D得到的愈伤组织不能分化。6-BA易于从嫩叶切片诱导胚性愈伤组织,进行体细胞胚发生过程的显微观察和代谢分析。6-BA诱导期间,DNA含量上升伴随胚性愈伤组织生长,水溶性蛋白变化稍延缓于RNA含量变化。培养第11天新出现12.13,13.08,66.30,97.25,104.31ku蛋白质,可能与胚性细胞出现有关,第25天新出现40.87ku蛋白质,与体细胞胚形成有关。Two types of explants, stem and leaf, from test tube seedlings of Phalaenopsis were cultured on the medium supplemented with 6-Benzylaminopurine（6-BA） and 2, 4 - dichloro phenoxyacetic acid（2,4-D） respectively. Embryogenic callus（EC）, whose cells contained large amount of starch grains, was induced with 6-BA from the stem, whereas the callus induced with 2,4-D was unable to differentiate. The EC was also induced efficiently from leaf explant cultured on the medium supplemented with 6-BA. Histological observation and metabolic analysis were carried on during the somatic embryogenesis. When the explants were induced with 6-BA, the DNA content in the explants was increased with the growth of the EC. The RNA content changed earlier than the soluble protein content. The SDS-PAGE profile of the soluble protein indicated that proteins （12.13 kDa,13.08 kDa, 66.30 kDa,97.25 kDa,104.31 kDa） came up at the 1 l th day of culture, which probably indicates the induction of embryo cells. And the protein of 40.87 kDa showed up at the 25th day of culture,indicating somatic embryogenesis.

关 键 词：蝴蝶兰 细胞分裂素 胚性愈伤组织 大分子代谢动态 

分 类 号：S682.31[农业科学—观赏园艺] Q813.12[农业科学—园艺学]