应用SRAP分子标记评价辣椒自交系的遗传关系  

SRAP Assessment of Genetic Relationship of Inbred Hot Pepper (Capsicum annuum L)

作　　者：任羽[1] 张银东[2] 尹俊梅[1] 王得元[3] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]中国热带农业科学院热带作物生物技术研究所,海口571101 [3]广东省农业科学院蔬菜研究所/广东省果蔬新技术研究重点实验室,广州510640

出　　处：《热带作物学报》2008年第1期47-52,共6页Chinese Journal of Tropical Crops

基　　金：广东省自然科学基金(000162);广州市重点科技攻关项目(2002Z2-E0192);广东省农业科学院院长基金项目(2001-基金-08)资助

摘　　要：将新型分子标记SRAP(Sequence-related amplified polymorphism,序列相关扩增多态性)应用于辣椒自交系的遗传关系的研究,采用30个引物组合对31个辣椒自交系进行扩增,其中有27个引物组合可以获得多态性扩增,显示了较高的多态性。27个引物组合共扩增出310个多态性条带,平均每个引物组合产生11.5个多态性条带。31个自交系间的相似系数为0.133～0.997。聚类分析将31个自交系分为:在相似系数D为0.55处,将辣椒的2个变种分开;在相似系数D为0.67处,将辣椒分为4类。结果显示,SRAP是辣椒自交系研究中一种较好标记,其分类结果有助于利用这些自交系进行杂交亲本的选配。Sequence-related amplified polymorphism （SRAP）, a new marker system, was applied in hot pepper genome analysis. SRAP marker was used to detect the polymorphisms in 31 inbred lines of hot pepper, of which 27 were found to show comparatively high polymorphism and generate 310 polymorphic bands with an average of 11.5 polymorphic bands per primer pair. The genetic similarities among the 31 inbred lines varied from 0.133 to 0.997. They were clustered into two groups and four groups at the level of D 0.55 and D 0.67, respectively. The results show that SRAP system is a good marker for study of hot pepper and facilitates selection of parents for crossing.

关 键 词：辣椒 SRAP 聚类分析 

分 类 号：S641.3[农业科学—蔬菜学] Q781[农业科学—园艺学]