鸡源大肠杆菌质粒介导的氟喹诺酮类药物耐药基因qnrA的分子检测  

Molecular Detection of the Plasmid-Mediated FQs Resistant qnrA Genes among Escherichia Coli Strains Isolated from Diseased Chickens

作　　者：杜向党[1] 李新生[1] 秦上尚[2] 刘建华[1] 张素梅[1] 沈建忠[2] 

机构地区：[1]河南农业大学牧医工程学院,郑州 450002 [2]中国农业大学动物医学院,北京 100094

出　　处：《中国畜牧兽医》2008年第5期28-30,共3页China Animal Husbandry & Veterinary Medicine

基　　金：“十一五”国家科技支撑计划(2006BAK02A03-3);河南农业大学青年科技创新基金项目资助

摘　　要：对2005年—2007年从豫北地区临床分离的377株鸡源大肠杆菌进行生化鉴定，MIC值测定。被测菌株对氟喹诺酮类（FQs）药物恩诺沙星、环丙沙星和诺氟沙星均呈严重耐药，耐药率分别为94．9％、93．9％、94．9％。选取对恩诺沙星耐药（MIC〉32μg／m1）的235株大肠杆菌进行qnr基因的分子检测，结果显示仅有1株大肠杆菌（MIC=128μg／m1）呈qnr基因阳性，经测序分析该基因命名为qnrA。377 isolates recovered from diseased chickens in northern region of Henan province from the year 2005 to 2007,which were conformed as escherichia coli strains by the biochemical test, were tested for antimicrobial susceptibility. All isolates displayed high resistance against FQs. The rates of resistance against enrofloxacin, ciprofloxacin, norfloxacin were 94.9 %, 93.9%, 94.9 %, respectively. 235 isolates which displayed high resistance against enrofloxacin （MIC）32 μg/ml） were further screened for the presence of the qnr genes using a PCR based strategy. The results showed that there was only one strain in which qnr-like genes was positive among these detected strains. This qnr-like gene was named qnrA after sequencing analysis.

关 键 词：大肠杆菌 氟喹诺酮类 耐药性 QNR基因 

分 类 号：S852.612[农业科学—基础兽医学]