甲醛吸入对小鼠学习记忆能力的影响  

Effect of Formaldehyde Inhalation on Mice's Learning and Memory

作　　者：王小玲[1] 原福胜[1] 张志红[1] 白剑英[1] 赵五红[1] 梁瑞峰[1] 

机构地区：[1]山西医科大学公共卫生学院环境卫生学教研室,山西太原030001

出　　处：《环境与健康杂志》2008年第5期400-402,共3页Journal of Environment and Health

基　　金：山西省自然科学基金资助项目(20031098)

摘　　要：目的研究甲醛吸入对小鼠学习记忆能力的影响。方法选择健康清洁级昆明种小鼠24只,雌雄各半,随机分为对照组(清洁空气)、甲醛低(1mg/m3)、中(3mg/m3)、高(5mg/m3)剂量组,每组6只。采用静式甲醛吸入染毒,每天2h,连续14d。染毒结束后,采用跳台实验、避暗实验及Morris水迷宫实验对小鼠进行神经行为学测试,检测其学习和记忆能力。结果随着甲醛染毒剂量的增加,小鼠体重增长缓慢。跳台实验结果显示,与对照组比较,中、高剂量组小鼠错误次数增多,从平台跳下的潜伏期缩短,差异有统计学意义(P<0.05)。避暗实验结果显示,与对照组比较,中、高剂量组小鼠错误次数增多,由明箱进入暗箱的潜伏期缩短,差异有统计学意义(P<0.05)。Morris水迷宫实验结果显示,高剂量组小鼠逃避潜伏期长于对照组,差异有统计学意义(P<0.05)。结论吸入较高剂量甲醛可导致小鼠的学习记忆能力下降,甲醛对小鼠中枢神经系统具有一定的毒性作用。Objective To study the effect of inhaling formaldehyde on learning and memory in mice. Methods 24 KM mice were randomly divided into four groups： the control group （clean air）, the low dose group （1 mg/m^3）, the moderate dose group （3 mg/m^3） and the high dose group （5 mg/m^3）. The mice were exposed to different concentrations ofgaseity formaldehyde, 2 h/day, for 14 consecutive days. Then their behavior of learning and memory were tested by step-down test, passive avoidance test and Morris water maze experiment. Results Step-down test showed that the mice in the moderate dose group and the high dose group made notable errors compared with the control group （P〈0.05） and the incubation period （jumping from the platform） significantly reduced （P〈0.05）. Passive avoidance test showed that compared with the control group, mice in the moderate dose group and the high dose group made notable errors（P〈0.05） and the incubation period （entering in dark chamber from light chamber） significantly reduced（P〈0.05）. Morris water maze test indicated that the incubation period of finding platform significantly extended in the high dose group（P〈0.05）. Conclusion Inhaling formaldehyde can cause learning and memory abilities decline in mice. Formaldehyde has obvious toxic effects on central nervous system in mice.

关 键 词：甲醛 记忆 学习 动物实验 

分 类 号：R994.6[医药卫生—毒理学]