叶下株提取物对盐酸氮芥损伤睾丸组织N-钙粘蛋白表达的保护作用  

Phyllanthus Urinaria Extract Promotes N-cadherin Expression in Nitrogen Mustard-disrupted Testicular Tissues in vivo

作　　者：张德迎[1] 何大维[1] 魏光辉[1] 刘星[1] 林涛[1] 李旭良[1] 

机构地区：[1]重庆医科大学附属儿童医院泌尿外科,重庆400014

出　　处：《中华男科学杂志》2008年第5期396-400,共5页National Journal of Andrology

基　　金：重庆市教委科研基金资助(KJ050305)

摘　　要：目的:研究叶下珠(PU)提取物对盐酸氮芥(HN2)损伤睾丸组织N-钙粘蛋白表达的保护作用。方法:以5mg/kg高剂量HN2腹腔注射雄性KM小鼠制备生殖毒性模型,48只KM小鼠随机分为正常对照组、高剂量HN2组(5mg/kg)、高剂量HN2+低剂量PU(125mg/kg)干预组、高剂量HN2+中剂量PU(250mg/kg)干预组、高剂量HN2+高剂量PU(500mg/kg)干预组、高剂量PU组(500mg/kg)6组,每组8只。分别应用免疫组化染色、RT-PCR、Western印迹检测各组小鼠睾丸组织N-钙粘蛋白分布、mRNA及蛋白质表达水平变化。结果:N-钙粘蛋白主要分布在生精小管基底部Sertoli细胞及Leydig细胞、管周细胞胞膜及胞质;HN2处理后,N-钙粘蛋白仅在生精小管基底部及管周细胞有少许表达;不同剂量PU干预高剂量HN2处理后,睾丸内N-钙粘蛋白mRNA和蛋白质表达随PU干预剂量增加而明显上调(P<0.01);高剂量PU干预HN2组及高剂量PU组,N-钙粘蛋白分布表达与正常睾丸组织无明显差异(P>0.05)。结论:PU能有效干预HN2对睾丸组织N-钙粘蛋白表达的损伤。Objective： To explore the protective effect of the PhyUanthus Urinaria （PU） extract on the N-cadherin expression in the testicular tissues disrupted by nitrogen mustard （ HN2） in vivo. Methods ： HN2 was intraperitoneally injected into male KM mice at the dose of 5 mg/kg to make reproductive toxicity models, and at the same time PU was administered for intervention at the dose of 125 mg/kg, 250 mg/kg and 500 mg/kg. N-cadherin distribution, mRNA and protein expression in the testicular tissues were detected by immunohistochemistry, RT-PCR and Western blotting. Results： N-cadherin was mainly distributed in the membrane and cytoplasm of Sertoli cells at the basement of seminiferous epithelia, Leyding cells and peritubular cells, scarcely expressed in the basement of seminiferous epithelia and peritubular cells after HN2 administration. The expressions of mRNA and proteins of N-cadherin were significantly elevated with the increased dose of PU （P 〈 0.01 ）. Compared with the normal control, the distribution and expression of N-cadherin showed no significant differences in either the high-dose PU group or the HN2 with high-dose PU intervention group （P 〉 0.05 ）.Conclusion ： The PU extract can effectively promote the N-cadherin expression in the testis tissues disrupted by HN2.

关 键 词：盐酸氮芥 叶下珠 睾丸 钙粘蛋白 

分 类 号：R285[医药卫生—中药学]