载脂蛋白A4基因多态性对中药降血脂的影响  

Effects of single nucleotide polymorphism of ApoA4 gene on blood lipid regulated by traditional Chinese medicine

作　　者：赵广荣[1] 肖敏[1] 李欲来[2] 张军平[2] 

机构地区：[1]天津大学化工学院制药工程系,天津300072 [2]天津中医药大学

出　　处：《中国老年学杂志》2008年第10期969-971,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30371712)

摘　　要：目的探讨载脂蛋白(Apo)A4基因多态性与芪参益气滴丸治疗气虚血瘀型冠心病疗效的关系。方法气虚血瘀型冠心病患者49例,门诊服用芪参益气滴丸1个月,治疗前后各抽取血样一次,测定血脂含量并提取外周血白细胞DNA,PCR扩增ApoA4基因第3外显子,测序检测多态性位点。结果在ApoA4基因的第3外显子中发现1个有义突变位点,第917位碱基C→T的突变,减弱了药物对LDL的降低作用,在非突变组比突变组之间达到极显著差异(P=0.009)。结论ApoA4基因C917T多态性对芪参益气滴丸降低低密度脂蛋白(LDL)有显著影响。Objective To explore the relationship between the polymorphism of ApoA4 gene and efficacy of Qishen benefiting vital energy drop pill on qi asthenia blood stasis coronary heart disease （CHD）. Methods Total 49 qi asthenia blood stasis CHD patients taken Qishen benefiting vital energy drop pill for 1 month were sampled to obtain the blood specimen before and after treatment to detect the content of blood fat and extract DNA from leucocyte of the blood samples, and amplify the third extron of ApoA4 gene by polymerase chain reaction. The polymorphic site was detected by sequencing. Results A plus sense mutant site APOA4 C917T was found in the third extron of ApoA4 gene, and the efficacy of reducing LDL in the nonmutant group was higher than that in the mutant group （P =0. 009 〈0. 01 ）. Conclusions The nonsynonymous mutant site APOA4 C917T can significantly affect the decreasing efficacy of Qishen benefiting vital energy drop pill on low density lipoprotein （LDL） content.

关 键 词：载脂蛋白A4 单核苷酸多态性 芪参益气滴丸 气虚血瘀型冠心病 

分 类 号：R932[医药卫生—生药学]